Cardioprotective role of Syzygium cumini against glucose-induced oxidative stress in H9C2 cardiac myocytes.

Abstract

Diabetic patients are known to have an independent risk of cardiomyopathy. Hyperglycemia leads to upregulation of reactive oxygen species (ROS) that may contribute to diabetic cardiomyopathy. Thus, agents that suppress glucose-induced intracellular ROS levels can have therapeutic potential against diabetic cardiomyopathy. Syzygium cumini is well known for its anti-diabetic potential, but its cardioprotective properties have not been evaluated yet. The aim of the present study is to analyze cardioprotective properties of methanolic seed extract (MSE) of S. cumini in diabetic in vitro conditions. ROS scavenging activity of MSE was studied in glucose-stressed H9C2 cardiac myoblasts after optimizing the safe dose of glucose and MSE by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide. 2',7'-dichlorfluorescein diacetate staining and Fluorescence-activated cell sorting analysis confirmed the suppression of ROS production by MSE in glucose-induced cells. The intracellular NO and H2O2 radical-scavenging activity of MSE was found to be significantly high in glucose-induced cells. Exposure of glucose-stressed H9C2 cells to MSE showed decline in the activity of catalase and superoxide dismutase enzymes and collagen content. 4',6-diamidino-2-phenylindole, propidium iodide and 10-N-nonyl-3,6-bis (dimethylamino) acridine staining revealed that MSE protects myocardial cells from glucose-induced stress. Taken together, our findings revealed that the well-known anti-diabetic S. cumini can also protect the cardiac cells from glucose-induced stress.

• Authors: Neha Atale, Mainak Chakraborty, Sujata Mohanty, Susinjan Bhattacharya, Darshika Nigam, Manish Sharma, Vibha Rani
• Journal: Cardiovascular toxicology (Cardiovasc Toxicol) Vol. 13 Issue 3 Pg. 278-89 (Sep 2013) ISSN: 1559-0259 [Electronic] United States
• PMID: 23512199 (Publication Type: Journal Article)

Chemical References

• Cardiotonic Agents
• Coloring Agents
• Free Radical Scavengers
• Plant Extracts
• Reactive Oxygen Species
• Solvents
• Nitric Oxide
• Hydrogen Peroxide
• Catalase
• Superoxide Dismutase
• Glucose
• Methanol

Topics

• Cardiotonic Agents
• Catalase (metabolism)
• Cell Line
• Cell Survival (drug effects)
• Coloring Agents
• Flow Cytometry
• Free Radical Scavengers
• Glucose (antagonists & inhibitors, toxicity)
• Humans
• Hydrogen Peroxide (metabolism)
• Membrane Potential, Mitochondrial (drug effects)
• Methanol
• Myocytes, Cardiac (drug effects)
• Nitric Oxide (metabolism)
• Oxidative Stress (drug effects)
• Plant Extracts (pharmacology)
• Reactive Oxygen Species (metabolism)
• Seeds (chemistry)
• Solvents
• Superoxide Dismutase (metabolism)
• Syzygium (chemistry)