Human platelet antigen genotyping and expression of CD109 (human platelet antigen 15) mRNA in various human cell types.

Abstract

CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34- PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.

• Authors: Sang Mee Hwang, Mi Jung Kim, Ho Eun Chang, Yun Ji Hong, Taek Soo Kim, Eun Young Song, Kyoung Un Park, Junghan Song, Kyou-Sup Han
• Journal: BioMed research international (Biomed Res Int) Vol. 2013 Pg. 946403 ( 2013) ISSN: 2314-6141 [Electronic] United States
• PMID: 23509816 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Antigens, Human Platelet
• CD109 protein, human
• GPI-Linked Proteins
• Neoplasm Proteins
• RNA, Messenger

Topics

• Antigens, CD (genetics)
• Antigens, Human Platelet (genetics)
• B-Lymphocytes (metabolism)
• Embryonic Stem Cells (cytology)
• Fibroblasts (cytology, metabolism)
• GPI-Linked Proteins (genetics)
• Gene Expression Profiling
• Gene Expression Regulation
• Genotype
• Granulocytes (metabolism)
• Humans
• K562 Cells
• Killer Cells, Natural (metabolism)
• Neoplasm Proteins (genetics)
• RNA, Messenger (genetics)
• Stem Cells (metabolism)