Increased levels of inosine in a mouse model of inflammation.

Abstract

One possible mechanism linking inflammation with cancer involves the generation of reactive oxygen, nitrogen, and halogen species by activated macrophages and neutrophils infiltrating sites of infection or tissue damage, with these chemical mediators causing damage that ultimately leads to cell death and mutation. To determine the most biologically deleterious chemistries of inflammation, we previously assessed products across the spectrum of DNA damage arising in inflamed tissues in the SJL mouse model nitric oxide overproduction ( Pang et al. ( 2007 ) Carcinogenesis 28 , 1807 - 1813 ). Among the anticipated DNA damage chemistries, we observed significant changes only in lipid peroxidation-derived etheno adducts. We have now developed an isotope-dilution, liquid chromatography-coupled, tandem quadrupole mass spectrometric method to quantify representative species across the spectrum of RNA damage products predicted to arise at sites of inflammation, including nucleobase deamination (xanthosine and inosine), oxidation (8-oxoguanosine), and alkylation (1,N(6)-ethenoadenosine). Application of the method to the liver, spleen, and kidney from the SJL mouse model revealed generally higher levels of oxidative background RNA damage than was observed in DNA in control mice. However, compared to control mice, RcsX treatment to induce nitric oxide overproduction resulted in significant increases only in inosine and only in the spleen. Further, the nitric oxide synthase inhibitor, N-methylarginine, did not significantly affect the levels of inosine in control and RcsX-treated mice. The differences between DNA and RNA damage in the same animal model of inflammation point to possible influences from DNA repair, RcsX-induced alterations in adenosine deaminase activity, and differential accessibility of DNA and RNA to reactive oxygen and nitrogen species as determinants of nucleic acid damage during inflammation.

• Authors: Erin G Prestwich, Aswin Mangerich, Bo Pang, Jose L McFaline, Pallavi Lonkar, Matthew R Sullivan, Laura J Trudel, Koli Taghizedeh, Peter C Dedon
• Journal: Chemical research in toxicology (Chem Res Toxicol) Vol. 26 Issue 4 Pg. 538-46 (Apr 15 2013) ISSN: 1520-5010 [Electronic] United States
• PMID: 23506120 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Nitric Oxide
• Inosine
• RNA
• DNA

Topics

• Animals
• Chromatography, Liquid
• DNA (metabolism)
• DNA Damage
• Disease Models, Animal
• Genetic Phenomena
• Inflammation (metabolism)
• Inosine
• Kidney (metabolism)
• Liver (metabolism)
• Mice
• Nitric Oxide (metabolism)
• Oxidation-Reduction
• RNA (metabolism)
• Spleen (metabolism)
• Tandem Mass Spectrometry