Relation of activation-induced deaminase (AID) expression with antibody response to A(H1N1)pdm09 vaccination in HIV-1 infected patients.

Abstract

The relevance of CD4+T-cells, viral load and age in the immunological response to influenza infection and vaccination in HIV-1 infected individuals has previously been pointed out. Our study aimed at assessing, in the setting of 2009 A(H1N1)pdm09 influenza vaccination, whether quantification of activation-induced deaminase (AID) expression in blood B-cells may provide additional indications for predicting antibody response to vaccination in HIV-1 infected patients with similar CD4+T-cell counts and age. Forty-seven healthy controls, 37 ART-treated and 17 treatment-naïve HIV-1 infected patients were enrolled in the study. Blood was collected prior to A(H1N1)pdm09 vaccination and at 1, 3 and 6 months after vaccination. Antibody titers to A(H1N1)pdm09 vaccine were measured by hemagglutination inhibition (HI) assay while the mRNA expression levels of AID were measured by quantitative real time PCR. Upon B-cell activation in vitro, AID increase correlated to antibody response to the A(H1N1)pdm09 vaccine at 1 month after vaccination in all individuals. In addition, the maximum expression levels of AID were significantly higher in those individuals who still carried protective levels of A(H1N1)pdm09 antibodies after 6 months from vaccination. No correlation was found between CD4+T-cell counts or age at vaccination or HIV-1 viral load and levels of A(H1N1)pdm09 antibodies. Assessing AID expression before vaccination may be an additional useful tool for defining a vaccination strategy in immune-compromised individuals at risk of immunization failure.

• Authors: Alberto Cagigi, Simone Pensieroso, Nicolas Ruffin, Stefano Sammicheli, Rigmor Thorstensson, Qiang Pan-Hammarström, Bo Hejdeman, Anna Nilsson, Francesca Chiodi
• Journal: Vaccine (Vaccine) Vol. 31 Issue 18 Pg. 2231-7 (Apr 26 2013) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 23499520 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Viral
• Influenza Vaccines
• AICDA (activation-induced cytidine deaminase)
• Cytidine Deaminase

Topics

• Adult
• Antibodies, Viral (blood, immunology)
• Antibody Formation (immunology)
• B-Lymphocytes (immunology, virology)
• CD4 Lymphocyte Count
• Cytidine Deaminase (immunology)
• Female
• HIV Infections (immunology, virology)
• HIV-1
• Healthy Volunteers
• Hemagglutination Inhibition Tests
• Humans
• Influenza A Virus, H1N1 Subtype (immunology)
• Influenza Vaccines (administration & dosage, immunology)
• Influenza, Human (immunology, prevention & control, virology)
• Lymphocyte Activation (immunology)
• Male
• Middle Aged
• Vaccination
• Viral Load