Auraptene suppresses inflammatory responses in activated RAW264 macrophages by inhibiting p38 mitogen-activated protein kinase activation.

Abstract

SCOPE:

Inflammation plays a key role in obesity-related pathologies such as insulin resistance and type 2 diabetes. Hypertrophied adipocytes trigger the enhancement of macrophage infiltration and the release of various proinflammatory factors in obese adipose tissue. In this study, we examined whether auraptene, a citrus-fruit-derived compound, could suppress the production of inflammatory factors that mediate the interaction between adipocytes and macrophages.

METHODS AND RESULTS:

Experiments using a co-culture system of 3T3-L1 adipocytes and RAW264 macrophages showed that auraptene reduced the production of nitric oxide and tumor necrosis factor-α. In RAW264 macrophages, auraptene also suppressed the inflammation induced by either LPS or the conditioned medium derived from 3T3-L1 adipocytes. In addition, auraptene inhibited the phosphorylation of the p38 mitogen-activated protein kinase and suppressed the production of proinflammatory mediators in activated macrophages.

CONCLUSION:

Our findings indicate that auraptene exhibits anti-inflammatory properties by suppressing the production of inflammatory factors that mediate the interaction between adipocytes and macrophages, suggesting that auraptene is a valuable food-derived compound with a potential to attenuate chronic inflammation in adipose tissue and to improve obesity-related insulin resistance.

• Authors: Shan Lin, Shizuka Hirai, Tsuyoshi Goto, Tomoya Sakamoto, Nobuyuki Takahashi, Masamichi Yano, Takao Sasaki, Rina Yu, Teruo Kawada
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 57 Issue 7 Pg. 1135-44 (Jul 2013) ISSN: 1613-4133 [Electronic] Germany
• PMID: 23495198 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Anti-Inflammatory Agents
• Ccl2 protein, mouse
• Chemokine CCL2
• Coumarins
• Culture Media, Conditioned
• Lipopolysaccharides
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• p38 Mitogen-Activated Protein Kinases
• aurapten

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism)
• Adipose Tissue (drug effects, metabolism)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Line, Tumor
• Chemokine CCL2 (metabolism)
• Citrus (chemistry)
• Coculture Techniques
• Coumarins (pharmacology)
• Culture Media, Conditioned
• Fruit (chemistry)
• Inflammation (drug therapy, pathology)
• Insulin Resistance
• Lipopolysaccharides (adverse effects)
• Macrophages (drug effects, metabolism)
• Mice
• Nitric Oxide (antagonists & inhibitors, metabolism)
• Obesity (metabolism)
• Phosphorylation
• Plant Extracts (pharmacology)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors, biosynthesis)
• p38 Mitogen-Activated Protein Kinases (genetics, metabolism)