[Study on prohibition of high mobility group chromosomal protein N2 against human oral squamous cell carcinoma in vitro].

Abstract

OBJECTIVE:

Take human oral squamous cell carcinoma Tca8113 as experimental model, and study the anti oral squamous cell carcinoma activity of high mobility group chromosomal protein N2 (HMGN2) molecule.

METHODS:

Train a large number of recombinant human HMGN2 expression vector Escherichia coli BL21. HMGN2 was expressed under isopropyl-1-thio-beta-galactopyranoside (IPTG) induction and purified by B-PER GST Fusion Protein Purification Kit. A variety of concentrations HMGN2 were added to cell culture medium, cells were tested by MTT, Hoechst 33342 fluorescence staining, flow cytometry assay and Western-blot.

RESULTS:

MTT results proved that HMGN2 could significantly inhibit human oral squamous cell carcinoma Tca8113 growth. Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis.

CONCLUSION:

HMGN2 can promote apoptosis of oral squamous cell carcinoma cells.

• Authors: Xiaoqian Dong, Xiqian Liu, Yonghong Zhang, Ping Zhang, Libing Lu, Xiaoyu Li, Ping Huang, Yun Feng
• Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology (Hua Xi Kou Qiang Yi Xue Za Zhi) Vol. 31 Issue 1 Pg. 91-5 (Feb 2013) ISSN: 1000-1182 [Print] China
• PMID: 23484311 (Publication Type: Journal Article)

Chemical References

• High Mobility Group Proteins
• Recombinant Proteins

Topics

• Apoptosis
• Carcinoma, Squamous Cell
• Cell Proliferation
• High Mobility Group Proteins
• Humans
• In Vitro Techniques
• Mouth Neoplasms
• Recombinant Proteins