The present study examines the therapeutic efficacy of the administration of low-dose
cisplatin (cis) followed by exposure to extremely low-frequency magnetic field (ELF-MF), with an average intensity of 10 mT, on Ehrlich
carcinoma in vivo. The cytotoxic and genotoxic actions of this combination were studied using comet assay, mitotic index (MI), and the induction of micronucleus (MN). Moreover, the inhibition of
tumor growth was also measured. Treatment with
cisplatin and ELF-MF (group A) increased the number of damaged cells by 54% compared with 41% for mice treated with
cisplatin alone (group B), 20% for mice treated by exposure to ELF-MF (group C), and 9% for the control group (group D). Also the mitotic index decreased significantly for all treated groups (P < 0.001). The decrement percent for the treated groups (A, B, and C) were 70%, 65%, and 22%, respectively, compared with the control group (D). Additionally, the rate of
tumor growth at day 12 was suppressed significantly (P < 0.001) for groups A, B, and C with respect to group (D). These results suggest that ELF-MF enhanced the cytotoxic activity of
cisplatin and potentiate the benefit of using a combination of low-dose
cisplatin and ELF-MF in the treatment of Ehrlich
carcinoma.