Inhibition of AKT in human pancreatic, renal and colorectal cancer cells by four cardiac hormones.

Abstract

BACKGROUND:

Protein kinase-B (AKT) is a serine/threonine protein kinase that has a key role in cell proliferation and cancer cell invasiveness. Four cardiac peptide hormones, namely vessel dilator, atrial natriuretic peptide (ANP), kaliuretic peptide, and long-acting natriuretic peptide (LANP) have anticancer effects both in vitro and in vivo.

MATERIALS AND METHODS:

Four cardiac hormones were examined for their ability to inhibit AKT, measured with a solid-phase enzyme-linked immunosorbent assay (ELISA) in human colorectal, pancreatic, and renal cancer cells.

RESULTS:

Vessel dilator, kaliuretic peptide, ANP, and LANP maximally reduced the concentration of AKT by 47%, 45%, 52%, and 46% in human colorectal cancer cells (p<0.0001), by 60%, 61%, 64%, and 59% in human pancreatic carcinoma cells (p<0.0001), and by 31%, 32%, 31%, and 31% in renal adenocarcinoma cells (p<0.001).

CONCLUSION:

These four cardiac hormones are significant inhibitors of AKT in human cancer cells, as part of their anticancer mechanism(s) of action.

• Authors: William P Skelton 4th, Michelle Skelton, David L Vesely
• Journal: Anticancer research (Anticancer Res) Vol. 33 Issue 3 Pg. 785-90 (Mar 2013) ISSN: 1791-7530 [Electronic] Greece
• PMID: 23482745 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Peptide Fragments
• Protein Precursors
• atrial natriuretic factor precursor (79-98)
• atrial natriuretic factor prohormone (1-30), human
• atrial natriuretic factor prohormone (31-67)
• Atrial Natriuretic Factor
• Proto-Oncogene Proteins c-akt

Topics

• Adenocarcinoma (drug therapy, enzymology)
• Antineoplastic Agents (pharmacology)
• Atrial Natriuretic Factor (pharmacology)
• Cell Line, Tumor
• Colorectal Neoplasms (drug therapy, enzymology)
• Humans
• Kidney Neoplasms (drug therapy, enzymology)
• Pancreatic Neoplasms (drug therapy, enzymology)
• Peptide Fragments (pharmacology)
• Protein Precursors (pharmacology)
• Proto-Oncogene Proteins c-akt (antagonists & inhibitors)