Transcript levels of major MMPs and ADAMTS-4 in relation to the clinicopathological profile of patients with tuberculous intervertebral discs and healthy controls.

Abstract

OBJECTIVES:

The purpose of the present study was to simultaneously examine the transcript levels of a large number of MMPs (-3, -8, -9, -13 and -14) and ADAMTS-4 and to investigate their correlation with the clinicopathological profile of patients suffering from tuberculous intervertebral discs.

DESIGN AND METHODS:

Clinical data were collected from 130 patients participating in the study from March 2011 to April 2012. mRNA expression levels were determined by means of the real-time polymerase chain reaction in 60 tuberculous (TB), 60 herniated, and 10 control intervertebral disc (ID) specimens.

RESULTS:

MMP-8, -9, -13, and -14 that showed a stronger expression in spinal TB disc tissue compared to normal ID tissue (P<0.05). Our results showed multiple positive correlations among MMP-8, -9, and -13 mRNA in TB samples. Smoking habits were found to significantly up-regulate the transcript levels of MMP-3 and -13 (P<0.05). Pain intensity, duration of symptoms, CRP, and ESR significantly affected the transcript levels of several MMPs (P<0.05).

CONCLUSIONS:

The MMPs may drive extracellular matrix destruction in spinal tuberculosis intervertebral discs. The experimental data imply a synergistic effect on the activity of these MMPs in spinal tuberculosis intervertebral discs. Furthermore, the experimental data suggest that smoking plays an unfavourable role in the prognosis of spinal tuberculosis intervertebral discs. Moreover, pain intensity, duration of symptoms, CRP, and ESR may affect the process of extracellular matrix destruction by increasing the expression of MMPs in spinal tuberculosis intervertebral disc samples.

• Authors: Chunxiang Xiong, Xinli Zhan, Zengming Xiao
• Journal: Clinical biochemistry (Clin Biochem) Vol. 46 Issue 7-8 Pg. 603-11 (May 2013) ISSN: 1873-2933 [Electronic] United States
• PMID: 23481488 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Chemical References

• ADAM Proteins
• Matrix Metalloproteinases
• Procollagen N-Endopeptidase
• ADAMTS4 Protein
• ADAMTS4 protein, human

Topics

• ADAM Proteins (biosynthesis)
• ADAMTS4 Protein
• Adult
• Aged
• Female
• Humans
• Intervertebral Disc Displacement (etiology, metabolism)
• Male
• Matrix Metalloproteinases (biosynthesis, genetics)
• Middle Aged
• Procollagen N-Endopeptidase (biosynthesis)
• Tuberculosis, Spinal (complications, metabolism)