Effects of mannan oligosaccharide on the physiological responses, HSP70 gene expression and disease resistance of Allogynogenetic crucian carp (Carassius auratus gibelio) under Aeromonas hydrophila infection.

Abstract

We evaluated the effect of dietary supplementation with mannan oligosaccharide (MOS) on the resistance to Aeromonas hydrophi1a infection in Allogynogenetic crucian carp. The fish were randomly divided into five groups: a control group was fed with basal diet, and four treatment groups fed with basal diet supplemented with 60, 120, 240, 480 mg/kg MOS for 10 weeks, respectively. We then challenged the fish with A. hydrophi1a and recorded the mortality and the changes in serum cortisol, T3, T4, lysozyme, alkaline phosphatase (ALP), globin and hepatic total anti-oxidative capacity, superoxide dismutase (SOD), malondialdehyde (MDA) and the relative expression of heat shock protein 70 (HSP70) mRNA for a period of 7 d. Supplementation with 240 mg/kg MOS significantly increased serum ALP activity before infection, 1d and 2d after infection, serum globin concentration prior to infection, 1d and 7d after infection, serum lysozyme activity at 2d after infection, T3 concentration at 2d after infection, hepatic total anti-oxidative capacity prior to infection, hepatic SOD activity at 7d after infection and reduced serum cortisol concentration at 2d after infection, hepatic malondialdehyde content at 1d and 2d after infection. Supplementation with 480 mg/kg MOS significantly increased serum ALP activity before infection, 1d and 2d after infection, T3 content 1d after infection, T4 content prior infection and 7d after infection, serum globin concentration prior to infection, 1d and 7d after infection, serum lysozyme activity prior infection and 1d after infection, serum total anti-oxidative capacity prior to infection and 7d after infection, hepatic SOD activity at 7d after infection and the relative level of hepatic HSP70 mRNA at 2d and 7d after infection, had decreased levels of serum cortisol concentration before the infection, at 2d after infection, T4 concentration at 1d and 2d after infection, hepatic malondialdehyde content at 1d and 2d after infection. Mortality was significantly lower in the group of 240 and 480 mg/kg MOS than the control. Our results suggest that ingestion of a basal diet supplemented with 240-480 mg/kg MOS can enhance resistance against pathogenic infections in Allogynogenetic crucian carp.

• Authors: Bo Liu, Lei Xu, Xianping Ge, Jun Xie, Pao Xu, Qunlan Zhou, Liangkun Pan, YuanYuan Zhang
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 34 Issue 6 Pg. 1395-403 (Jun 2013) ISSN: 1095-9947 [Electronic] England
• PMID: 23481213 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Fish Proteins
• HSP70 Heat-Shock Proteins
• Mannans

Topics

• Aeromonas hydrophila (immunology)
• Animal Feed (analysis)
• Animals
• Aquaculture
• Blood Chemical Analysis (veterinary)
• Carps
• Dietary Supplements (analysis)
• Disease Resistance
• Female
• Fish Diseases (genetics, immunology, microbiology)
• Fish Proteins (genetics, metabolism)
• Gene Expression Regulation
• Gram-Negative Bacterial Infections (genetics, immunology, microbiology, veterinary)
• HSP70 Heat-Shock Proteins (genetics, metabolism)
• Liver (metabolism)
• Mannans (administration & dosage)
• Oxidation-Reduction
• Saccharomyces cerevisiae (chemistry)