In the present study, we examined the liver
protein profiles of the large yellow croaker (Pseudosciaena crocea) exposed to
polyriboinosinic:polyribocytidylic acid [
poly(I:C)], a viral mimic, using the differential proteomic approach. Sixteen altered
protein spots were identified by matrix-assisted
laser desorption ionization time of flight mass spectrometry or matrix-assisted
laser desorption ionization time of flight/time of flight mass spectrometry, including eight upregulated
proteins and eight downregulated
proteins. These altered host
proteins were classified into six categories based on their
biological function: cellular process, metabolic process, biological regulation, binding, and catabolic process, highlighting the fact that response to
poly(I:C) induction in fish seems to be complex and diverse. Moreover, four corresponding genes of the differentially expressed
proteins were validated by relative quantitative real-time PCR. Western blot analysis further demonstrated the changes in
protein abundance of natural killer enhancing factor and
peroxiredoxin 6. These results will be helpful in furthering our understanding of the changes of physiological processes in liver of fish during
virus infection.