Duodenal-jejunal bypass improves glycemia and decreases SGLT1-mediated glucose absorption in rats with streptozotocin-induced type 2 diabetes.

To elucidate whether duodenal-jejunal-bypass (DJB), which improves blood glucose control, changes activity of Na-D-glucose cotransporter SGLT1 in small intestine.
DJB has been shown to improve oral glucose tolerance in normal rats and a genetic diabetic rat model. Because intestinal D-glucose absorption is mediated by SGLT1 localized in the brush border membrane of small intestinal enterocytes, it is unclear whether function of SGLT1 is altered by DJB and whether this contributes to the improvement of glycemic control.
A high-fat diet and low-dose streptozotocin administration were used to induce a type 2 diabetes in male Lewis rats. The diabetic animals underwent DJB or sham surgery. An oral glucose tolerance test (OGTT) was used to evaluate glucose control 3 weeks after surgery. SGLT1-mediated glucose transport was assessed using everted rings of different small intestinal segments. SGLT1 mRNA expression was determined by quantitative reverse transcription polymerase chain reaction (RT-PCR).
DJB improved the OGTT significantly (P < 0.001) compared with sham-operated rats while body weight was not different among the surgical groups. DJB induced a 50% reduction of SGLT1-mediated glucose uptake into enterocytes of duodenum and jejunum (P < 0.001). The concentration of D-glucose in the blood following glucose gavage increased more slowly after DJB versus sham.
The data indicate that DJB surgery decreases glucose absorption in the small intestine by downregulation of SGLT1-mediated glucose uptake. We suggest that the downregulation of SGLT1 contributes to the body-weight independent improvement of diabetes type 2.
AuthorsChristian Ferdinand Jurowich, Prashanth Reddy Rikkala, Andreas Thalheimer, Christian Wichelmann, Florian Seyfried, Victor Sander, Michael Kreissl, Christoph-Thomas Germer, Hermann Koepsell, Christoph Otto
JournalAnnals of surgery (Ann Surg) Vol. 258 Issue 1 Pg. 89-97 (Jul 2013) ISSN: 1528-1140 [Electronic] United States
PMID23478528 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Blood Glucose
  • C-Peptide
  • Slc5a1 protein, rat
  • Sodium-Glucose Transporter 1
  • Animals
  • Blood Glucose (metabolism)
  • C-Peptide (metabolism)
  • Diabetes Mellitus, Experimental (metabolism, surgery)
  • Duodenum (metabolism, surgery)
  • Enzyme-Linked Immunosorbent Assay
  • Glucose Tolerance Test
  • Jejunum (metabolism, surgery)
  • Male
  • Rats
  • Rats, Inbred Lew
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sodium-Glucose Transporter 1 (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: