Functional characterisation and transcript analysis of an alkaline phosphatase from the arbuscular mycorrhizal fungus Funneliformis mosseae.

Abstract	Alkaline phosphatases (ALP) in arbuscular mycorrhizal (AM) fungi have been suggested to be involved in transfer of phosphate from the mycorrhizal fungus to the host plant, but exact mechanisms are still unknown, partially due to the lack of molecular information. We isolated a full-length cDNA (FmALP) from the AM fungus Funneliformis mosseae (syn. Glomus mosseae) showing similarity with putative ALP genes from Rhizophagus intraradices (syn. Glomus intraradices) and Gigaspora margarita. For functional characterisation FmALP was expressed heterologously in the yeast Pichia pastoris. The recombinant FmALP protein had a pH optimum of 9.5, and catalysed the hydrolysis of glycerolphosphate and, to a lesser extent of glucose-1- and 6-phosphate, confirming it to be an alkaline phosphatase belonging to the family of alkaline phosphomonoesterases (EC 3.1.3.1). FmALP did not catalyse the hydrolysis of ATP or polyP. Relative FmALP transcript levels were analysed in intra- and extraradical hyphae isolated from F. mosseae infected ryegrass (Lolium perenne) using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). FmALP was highly expressed in intraradical hyphae at low P(i) supply, and its expression was repressed by high P(i) supply. Taken together this study provides evidence for mycorrhizal alkaline phosphatases playing a role in P mobilisation from organic substrates under P starvation conditions.
Authors	Qianhe Liu, Anthony J Parsons, Hong Xue, Chris S Jones, Susanne Rasmussen
Journal	Fungal genetics and biology : FG & B (Fungal Genet Biol) Vol. 54 Pg. 52-9 (May 2013) ISSN: 1096-0937 [Electronic] United States
PMID	23474124 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright © 2013 Elsevier Inc. All rights reserved.
Chemical References	Alkaline Phosphatase
Topics	Alkaline Phosphatase (chemistry, genetics, metabolism) Fungi (genetics, growth & development, metabolism) Hyphae (genetics, growth & development, metabolism) Mycorrhizae (genetics) Phylogeny Plant Roots (genetics, metabolism, microbiology) Sequence Analysis, DNA Symbiosis (genetics)

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