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MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia.

Abstract
Matrix metallinoprotease-9 (MMP9) plays a key role in the pathogenesis of post-ischemic blood brain barrier (BBB) disruption and the formation of lesions after cerebral ischemia. In this study we investigate the effect of brain-specific miRNAs on MMP-9 protein level in the rat hippocampus following cerebral ischemia and its underlying mechanism. Cerebral ischemia significantly upregulated miR-21 and -224 in the hippocampus; however, expression of miR-122 and -338-3p was not significantly affected by ischemia. Silencing of miR-21, but not -224, reduced MMP9 protein level after cerebral ischemia. Downregulation of extracellular signal-regulated kinase (ERK) signaling using the ERK inhibitor U0126 and the calcium-channel blocker ketamine inhibited the upregulation of miR-21 expression and MMP9 protein level after cerebral ischemia. The study suggests that cerebral ischemia up-regulates expression level of miR-21, which is involved in ERK-stimulated upregulation of MMP9 following cerebral ischemia via a calcium-dependent mechanism.
AuthorsXiaHeng Deng, Yun Zhong, LiZe Gu, Wei Shen, Jun Guo
JournalBrain research bulletin (Brain Res Bull) Vol. 94 Pg. 56-62 (May 2013) ISSN: 1873-2747 [Electronic] United States
PMID23473787 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier Inc. All rights reserved.
Chemical References
  • MicroRNAs
  • mirn21 microRNA, rat
  • Mmp9 protein, rat
  • Matrix Metalloproteinase 9
Topics
  • Animals
  • Blotting, Western
  • Brain Ischemia (metabolism)
  • Hippocampus (metabolism)
  • MAP Kinase Signaling System (physiology)
  • Male
  • Matrix Metalloproteinase 9 (biosynthesis)
  • MicroRNAs (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Real-Time Polymerase Chain Reaction
  • Up-Regulation

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