Transmissible spongiform encephalopathies (TSEs) or
prion diseases are associated with accumulations of disease specific PrP (
PrP(d)) in the central nervous system (CNS) and often the lymphoreticular system (LRS). Accumulations have additionally been recorded in other tissues including the peripheral nervous system and adrenal gland. Here we investigate the effect of sheep
scrapie on the morphology and the accumulation of
PrP(d) in the adrenal medulla of
scrapie affected sheep using light and electron microscopy. Using immunogold electron microscopy, non-fibrillar forms of
PrP(d) were shown to accumulate mainly in association with chromaffin cells, occasional nerve endings and macrophages.
PrP(d) accumulation was associated with distinctive membrane changes of chromaffin cells including increased electron density, abnormal linearity and invaginations. Internalisation of
PrP(d) from the chromaffin cell plasma membrane occurred in association with granule recycling following
hormone exocytosis.
PrP(d) accumulation and internalisation from membranes is similarly associated with perturbations of membrane structure and trafficking in CNS neurons and tingible body macrophages of the LRS. These data suggest that a major toxic effect of
PrP(d) is at the level of plasma membranes. However, the precise nature of
PrP(d)-membrane toxicity is tissue and cell specific suggesting that the normal
protein may act as a multi-functional scaffolding molecule. We further suggest that the co-localisation of
PrP(d) with exocytic granules of the
hormone trafficking system may provide an additional source of infectivity in blood.