γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Abstract

Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

• Authors: Jing-Shu Zhang, Da-Ming Li, Yue Ma, Ning He, Qing Gu, Feng-Shan Wang, Shu-Qing Jiang, Bing-Qing Chen, Jia-Ren Liu
• Journal: PloS one (PLoS One) Vol. 8 Issue 2 Pg. e57779 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 23469066 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Chromans
• Wnt Proteins
• Vitamin E
• plastochromanol 8
• Caspase 3

Topics

• Antineoplastic Agents (pharmacology)
• Caspase 3 (metabolism)
• Cell Death (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Chromans (pharmacology)
• Colonic Neoplasms (pathology)
• Enzyme Activation (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Signal Transduction (drug effects)
• Vacuoles (drug effects, metabolism)
• Vitamin E (analogs & derivatives, pharmacology)
• Wnt Proteins (genetics, metabolism)