Comparison of Kato-Katz, ethyl-acetate sedimentation, and Midi Parasep® in the diagnosis of hookworm, Ascaris and Trichuris infections in the context of an evaluation of rural sanitation in India.

Abstract

The Kato-Katz, conventional ethyl-acetate sedimentation, and Midi Parasep(®) methods for diagnosing infection with soil-transmitted helminths were compared. The Kato-Katz technique gave the best overall diagnostic performance with the highest results in all measures (prevalence, faecal egg count, sensitivity) followed by the conventional ethyl-acetate and then the Midi Parasep(®) technique. The Kato-Katz technique showed a significantly higher faecal egg count and sensitivity for both hookworm and Trichuris as compared to the Midi Parasep(®) technique. The conventional ethyl-acetate technique produced smaller pellets and showed lower pellet mobility as compared to the Midi Parasep(®).

• Authors: Anna L Funk, Sophie Boisson, Thomas Clasen, Jeroen H J Ensink
• Journal: Acta tropica (Acta Trop) Vol. 126 Issue 3 Pg. 265-8 (Jun 2013) ISSN: 1873-6254 [Electronic] Netherlands
• PMID: 23467010 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Topics

• Ancylostomatoidea (isolation & purification)
• Animals
• Ascariasis (diagnosis)
• Ascaris (isolation & purification)
• Clinical Laboratory Techniques (methods)
• Diagnostic Tests, Routine (methods)
• Hookworm Infections (diagnosis)
• Humans
• India
• Parasitology (methods)
• Rural Population
• Sensitivity and Specificity
• Trichuriasis (diagnosis)
• Trichuris (isolation & purification)