In vivo effects of tailored laminin-332 α3 conjugated scaffolds enhances wound healing: a histomorphometric analysis.

Abstract

Surface modification techniques have been used to develop biomimetic scaffolds by incorporating cell adhesion peptides. In our previous work, we have shown the tethering of laminin-332 α3 chain to type I collagen scaffold using microbial transglutaminase (mTGase), promotes cell adhesion, migration, and proliferation. In this study, we evaluated the wound healing properties of tailored laminin-332 α3 chain (peptide A: PPFLMLLKGSTR) tethered to a type I collagen scaffold using mTGase by incorporating transglutaminase substrate peptide sequences containing either glutamine (peptide B: PPFLMLLKGSTREAQQIVM) or lysine (peptide C: PPFLMLLKGSTRKKKKG) in rat full-thickness wound model at two different time points (7 and 21 days). Histological evaluations were assessed for wound closure, epithelialization, angiogenesis, inflammatory, fibroblastic cellular infiltrations, and quantified using stereological methods (p < 0.05). Peptide A and B tethered to collagen scaffold using mTGase stimulated neovascularization, decreased the inflammatory cell infiltration and prominently enhanced the fibroblast proliferation which significantly accelerated the wound healing process. We conclude that surface modification by incorporating motif of laminin-332 α3 chain (peptide A: PPFLMLLK GSTR) domain and transglutaminase substrate to the laminin-332 α3 chain (peptide B: PPFLMLLKGSTREAQQIVM) using mTGase may be a potential candidate for tissue engineering applications and skin regeneration.

• Authors: Gopinath Damodaran, William H C Tiong, Russell Collighan, Martin Griffin, Harshad Navsaria, Abhay Pandit
• Journal: Journal of biomedical materials research. Part A (J Biomed Mater Res A) Vol. 101 Issue 10 Pg. 2788-95 (Oct 2013) ISSN: 1552-4965 [Electronic] United States
• PMID: 23463686 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Wiley Periodicals, Inc., a Wiley Company.

Chemical References

• Cell Adhesion Molecules
• Fibrillar Collagens
• kalinin
• Transglutaminases

Topics

• Amino Acid Sequence
• Animals
• Cattle
• Cell Adhesion Molecules (chemistry, pharmacology)
• Cell Size (drug effects)
• Fibrillar Collagens (chemistry)
• Fibroblasts (drug effects, pathology)
• Inflammation (pathology)
• Male
• Molecular Sequence Data
• Neovascularization, Physiologic (drug effects)
• Rats
• Rats, Sprague-Dawley
• Tissue Scaffolds (chemistry)
• Transglutaminases (metabolism)
• Wound Healing (drug effects)