Abstract |
The mechanism of action of a novel anti-HIV antibiotic, pradimicin A, has been studied using cell-free (MT-4 cells) and cell to cell (MOLT-4 and MOLT-4/HIVHTLV-3B cells) HIV infection systems. The data indicate that (1) preincubation of the cells with HIV at 0 degrees for 1 hr, followed by the addition of pradimicin A and further incubation at 37 degrees, resulted in a complete inhibition of infection while preincubation at 37 degrees did not. Similar data were obtained with the coculture between MOLT-4 and MOLT-4/HIV cells. (2) Virus treated with pradimicin A followed by washing did not show any inhibition on subsequent HIV binding to cells. (3) The inhibitory effect of pradimicin A on HIV infection was prevented by mannan but not by other sugars tested. Mannan, however, did not interfere with the anti-HIV activity of other known inhibitors. (4) Use of EGTA suggested that pradimicin A required Ca ions to exert its anti-HIV activity. These data imply that pradimicin A inhibits an early step in HIV infection, probably through its binding to mannose residues of HIV glycoprotein.
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Authors | A Tanabe-Tochikura, T S Tochikura, O Yoshida, T Oki, N Yamamoto |
Journal | Virology
(Virology)
Vol. 176
Issue 2
Pg. 467-73
(Jun 1990)
ISSN: 0042-6822 [Print] United States |
PMID | 2345961
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anthracyclines
- Antibiotics, Antineoplastic
- Antiviral Agents
- Mannans
- pradimicin A
- Calcium
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Topics |
- Anthracyclines
- Antibiotics, Antineoplastic
(metabolism, pharmacology)
- Antiviral Agents
(metabolism, pharmacology)
- Calcium
(metabolism)
- Cell Line
- Cold Temperature
- Cytopathogenic Effect, Viral
- HIV
(drug effects)
- Humans
- Mannans
(metabolism, pharmacology)
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