β-cryptoxanthin regulates bone resorption related-cytokine production in human periodontal ligament cells.

Abstract

OBJECTIVE:

β-cryptoxanthin (β-cry) is a type of carotenoid found in certain fruits and vegetables. Although it has been shown that β-cry inhibits alveolar bone resorption, the molecular mechanisms for this have not yet been clarified. In the present study, we investigated the effects of β-cry on bone resorption related-cytokine production in human periodontal ligament (hPDL) cells.

DESIGN:

hPDL cells were stimulated with β-cry (1×10(-7)mol/l), mechanical stress (1 or 6MPa), and P. gingivalis. The production of interleukin (IL)-1β, IL-6, IL-8, tumour necrosis factor (TNF)-α, osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) were analyzed by RT-PCR and ELISA.

RESULTS:

The production of IL-1β, IL-6, IL-8, and TNF-α was not induced in hPDL cells after stimulation with β-cry, although these cytokines were produced after stimulation with P. gingivalis. On the other hand, IL-6 and IL-8 were produced after exposure to 6MPa of mechanical stress. The production of IL-6 and IL-8 was significantly decreased by the addition of β-cry. Furthermore, β-cry up-regulated the production of OPG, but not RANKL.

CONCLUSION:

β-cry inhibited the production of IL-6 and IL-8 induced by mechanical stress and periodontopathogenic bacteria in hPDL cells. Moreover, β-cry up-regulated OPG production. These results suggest that β-cry may prevent bone resorption in periodontitis.

• Authors: Masaru Nishigaki, Toshiro Yamamoto, Hiroaki Ichioka, Ken-Ichi Honjo, Kenta Yamamoto, Fumishige Oseko, Masakazu Kita, Osam Mazda, Narisato Kanamura
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 58 Issue 7 Pg. 880-6 (Jul 2013) ISSN: 1879-1506 [Electronic] England
• PMID: 23452546 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Cryptoxanthins
• Cytokines
• Osteoprotegerin
• RNA, Messenger
• Xanthophylls

Topics

• Bacteroidaceae Infections
• Bone Resorption (prevention & control)
• Cells, Cultured
• Cryptoxanthins
• Cytokines (biosynthesis, genetics)
• Enzyme-Linked Immunosorbent Assay
• Gene Expression
• Humans
• Osteoprotegerin (biosynthesis)
• Periodontal Ligament (cytology, drug effects)
• Periodontitis (physiopathology)
• Porphyromonas gingivalis (pathogenicity)
• RNA, Messenger (biosynthesis)
• Reverse Transcriptase Polymerase Chain Reaction
• Stress, Mechanical
• Up-Regulation
• Xanthophylls (pharmacology)