Anticancer activity of olive oil hydroxytyrosyl acetate in human adenocarcinoma Caco-2 cells.

Abstract

The anticancer activity of hydroxytyrosyl acetate (HTy-Ac) has been studied in human colon adenocarcinoma cells. Gene expression of proteins involved in cell cycle (p21, p53, cyclin B1, and cyclin G2) and programmed cell death (BNIP3, BNIP3L, PDCD4, and ATF3), as well as phase I and phase II detoxifying enzymes CYPA1 and UGT1A10, were evaluated by reverse transcription polymerase chain reaction after 24 h of exposure of Caco-2/TC7 cells to 5, 10, and 50 μM of HTy-Ac. The results show that HTy-Ac inhibited cell proliferation and arrested cell cycle by enhancing p21 and CCNG2 and lowering CCNB1 protein expression. HTy-Ac also affected the transcription of genes involved in apoptosis up-regulating of BNIP3, BNIP3L, PDCD4, and ATF3 and activating caspase-3. In addition, HTy-Ac also up-regulated xenobiotic metabolizing enzymes CYP1A1 and UGT1A10, thus enhancing carcinogen detoxification. In conclusion, these results highlight that HTy-Ac has the potential to modulate biomarkers involved in colon cancer.

• Authors: Raquel Mateos, Gema Pereira-Caro, James R Bacon, Roy Bongaerts, Beatriz Sarriá, Laura Bravo, Paul A Kroon
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 61 Issue 13 Pg. 3264-9 (Apr 03 2013) ISSN: 1520-5118 [Electronic] United States
• PMID: 23452288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetates
• Antineoplastic Agents
• Catechols
• DNA Primers
• Olive Oil
• Plant Oils
• hydroxytyrosyl acetate

Topics

• Acetates (pharmacology)
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Base Sequence
• Caco-2 Cells
• Catechols (pharmacology)
• Cell Proliferation (drug effects)
• DNA Primers
• Flow Cytometry
• Humans
• Olive Oil
• Plant Oils (chemistry)
• Real-Time Polymerase Chain Reaction