Abstract |
Miniature inverted-repeat transposable elements (MITEs) are widespread in both prokaryotic and eukaryotic genomes, where their copy numbers can attain several thousands. Little is known, however, about the genetic factor(s) affecting their transpositions. Here, we show that disruption of a gene encoding ubiquitin-like protein markedly enhances the transposition activity of a MITE mPing in intact rice plants without any exogenous stresses. We found that the transposition activity of mPing is far higher in the lines harboring a non-functional allele at the Rurm1 (Rice ubiquitin-related modifier-1) locus than in the wild-type line. Although the alteration of cytosine methylation pattern triggers the activation of transposable elements under exogenous stress conditions, the methylation degrees in the whole genome, the mPing-body region, and the mPing-flanking regions of the non-functional Rurm1 line were unchanged. This study provides experimental evidence for one of the models of genome shock theory that genetic accidents within cells enhance the transposition activities of transposable elements.
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Authors | Takuji Tsukiyama, Shota Teramoto, Kanako Yasuda, Akira Horibata, Nanako Mori, Yutaka Okumoto, Masayoshi Teraishi, Hiroki Saito, Akiko Onishi, Kanako Tamura, Takatoshi Tanisaka |
Journal | Molecular plant
(Mol Plant)
Vol. 6
Issue 3
Pg. 790-801
(May 2013)
ISSN: 1752-9867 [Electronic] England |
PMID | 23446031
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Transposable Elements
- Ubiquitin
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Topics |
- Base Sequence
- Crosses, Genetic
- DNA Methylation
(genetics)
- DNA Transposable Elements
(genetics)
- Gene Dosage
- Genes, Plant
(genetics)
- Molecular Sequence Data
- Mutagenesis, Insertional
(genetics)
- Oryza
(anatomy & histology, genetics)
- Phenotype
- Polymerase Chain Reaction
- Ubiquitin
(metabolism)
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