Abstract |
The aim of this study was to investigate the cytotoxicity of paclitaxel solid lipid nanoparticles (SLN) modified with stearic acid octaarginine (SA-R8-PTX-SLN) as well as the cellular uptake of coumarin-6-loaded SLN modified with SA-R8 (SA-R8-C6-SLN) in human lung cancer cells, A549. SLN were prepared using a film dispersion method; and then their particle size, zeta potential, morphology, bound efficiency of SAR8, drug loading efficiency, and in vitro release were characterized. SA-R8-PTX-SLN and SA-R8-C6-SLN were incubated with A549 cells to measure their cytotoxicity and cellular uptake, respectively. The results indicated that the cytotoxicity of SA-R8-PTX-SLN was enhanced significantly with the increasing amount of SA-R8 and the cellular uptakes of SLN increased with the incubated concentrations and the incubated time of SLN. In contrast, SA-R8-SLN could significantly enhance the cellular uptake of SLN and the cytotoxicity of PTX in A549 cells. These in vitro results suggest that SA-R8-SLN could be proposed as alternative drug delivery system.
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Authors | Yin-Long Zhang, Zhen-Hai Zhang, Tian-Yue Jiang, Ayman-Waddad, Jing-Li, Hui-Xia Lv, Jian-Ping Zhou |
Journal | Die Pharmazie
(Pharmazie)
Vol. 68
Issue 1
Pg. 47-53
(Jan 2013)
ISSN: 0031-7144 [Print] Germany |
PMID | 23444780
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Coumarins
- Lipids
- Peptides
- coumarin
- Paclitaxel
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Topics |
- Antineoplastic Agents, Phytogenic
(administration & dosage, chemistry, pharmacokinetics)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Coumarins
(pharmacokinetics)
- Dialysis
- Dose-Response Relationship, Drug
- Humans
- Lipids
(chemistry)
- Microscopy, Atomic Force
- Microscopy, Fluorescence
- Nanoparticles
- Paclitaxel
(administration & dosage, chemistry, pharmacokinetics)
- Particle Size
- Peptides
(chemistry, pharmacology)
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