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Cell uptake of paclitaxel solid lipid nanoparticles modified by cell-penetrating peptides in A549 cells.

Abstract
The aim of this study was to investigate the cytotoxicity of paclitaxel solid lipid nanoparticles (SLN) modified with stearic acid octaarginine (SA-R8-PTX-SLN) as well as the cellular uptake of coumarin-6-loaded SLN modified with SA-R8 (SA-R8-C6-SLN) in human lung cancer cells, A549. SLN were prepared using a film dispersion method; and then their particle size, zeta potential, morphology, bound efficiency of SAR8, drug loading efficiency, and in vitro release were characterized. SA-R8-PTX-SLN and SA-R8-C6-SLN were incubated with A549 cells to measure their cytotoxicity and cellular uptake, respectively. The results indicated that the cytotoxicity of SA-R8-PTX-SLN was enhanced significantly with the increasing amount of SA-R8 and the cellular uptakes of SLN increased with the incubated concentrations and the incubated time of SLN. In contrast, SA-R8-SLN could significantly enhance the cellular uptake of SLN and the cytotoxicity of PTX in A549 cells. These in vitro results suggest that SA-R8-SLN could be proposed as alternative drug delivery system.
AuthorsYin-Long Zhang, Zhen-Hai Zhang, Tian-Yue Jiang, Ayman-Waddad, Jing-Li, Hui-Xia Lv, Jian-Ping Zhou
JournalDie Pharmazie (Pharmazie) Vol. 68 Issue 1 Pg. 47-53 (Jan 2013) ISSN: 0031-7144 [Print] Germany
PMID23444780 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Coumarins
  • Lipids
  • Peptides
  • coumarin
  • Paclitaxel
Topics
  • Antineoplastic Agents, Phytogenic (administration & dosage, chemistry, pharmacokinetics)
  • Cell Line, Tumor
  • Cell Survival (drug effects)
  • Coumarins (pharmacokinetics)
  • Dialysis
  • Dose-Response Relationship, Drug
  • Humans
  • Lipids (chemistry)
  • Microscopy, Atomic Force
  • Microscopy, Fluorescence
  • Nanoparticles
  • Paclitaxel (administration & dosage, chemistry, pharmacokinetics)
  • Particle Size
  • Peptides (chemistry, pharmacology)

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