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Reduction in VLDL, but not HDL, in plasma of rats deficient in choline.

Abstract
We have analyzed plasma lipoprotein levels in young male rats fed a choline-deficient diet for 3 days. We confirmed previous studies that choline deficiency promotes 6.5-fold accumulation of triacyglycerol in the liver (23.9 +/- 6.0 versus 3.69 +/- 0.92 mumol/g liver) and reduction of triacylglycerol concentration in plasma by 60% (0.17 +/- 0.04 versus 0.46 +/- 0.10 mumol/mL plasma). Agarose gel electrophoresis showed that the plasma very low density lipoprotein (VLDL) levels were reduced in choline-deficient rats, but the concentration of plasma high density lipoproteins (HDL) was not affected. Sodium dodecyl sulfate - polyacrylamide gel electrophoresis of fractionated plasma lipoproteins revealed that the concentrations of apolipoproteins (apo) BH, BL, and E in VLDL from choline-deficient rats were 37.1, 11.0, and 37.2% of normal levels, respectively. In contrast, the amount of apo A-I, the major one in HDL, was almost unchanged. Correspondingly, there were decreased lipid (mainly phosphatidylcholine and triacylglycerol) levels in VLDL from choline-deficient rats, but no change in the levels of phosphatidylcholine, cholesterol, and cholesterol ester in HDL. There were similar levels of apo B and E (components of VLDL) in homogenates of livers from normal and choline-deficient rats, as determined by immunoblotting. These results support the hypothesis that choline deficiency causes reduction of VLDL, but not HDL, levels in plasma as a consequence of impaired hepatic VLDL secretion.
AuthorsZ M Yao, D E Vance
JournalBiochemistry and cell biology = Biochimie et biologie cellulaire (Biochem Cell Biol) Vol. 68 Issue 2 Pg. 552-8 (Feb 1990) ISSN: 0829-8211 [Print] Canada
PMID2344402 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Apolipoproteins
  • Lipids
  • Lipoproteins, HDL
  • Lipoproteins, VLDL
Topics
  • Animals
  • Apolipoproteins (blood)
  • Choline Deficiency (blood, metabolism)
  • Lipid Metabolism
  • Lipids (blood)
  • Lipoproteins, HDL (blood)
  • Lipoproteins, VLDL (blood, metabolism)
  • Liver (metabolism)
  • Male
  • Rats
  • Rats, Inbred Strains

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