Expression of Bacteroides fragilis hemolysins in vivo and role of HlyBA in an intra-abdominal infection model.

Bacteroides fragilis is the most frequent opportunistic pathogen isolated from anaerobic infections. However, there is a paucity of information regarding the genetic and molecular aspects of gene expression of its virulence factors during extra-intestinal infections. A potential virulence factor that has received little attention is the ability of B. fragilis to produce hemolysins. In this study, an implanted perforated table tennis "ping-pong" ball was used as an intra-abdominal artificial abscess model in the rat. This procedure provided sufficient infected exudate for gene expression studies in vivo. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to quantify the relative expression of hlyA, hlyB, hlyC, hlyD, hlyE, hlyF, hlyG, and hlyIII mRNAs. The hlyA mRNA was induced approximately sixfold after 4 days postinfection compared with the mRNA levels in the inoculum culture prior to infection. The hlyB mRNA increased approximately sixfold after 4 days and 12-fold after 8 days postinfection. Expression of hlyC mRNA increased sixfold after 1 day, 45-fold after 4 days, and 16-fold after 8 days postinfection, respectively. The hlyD and hlyE mRNAs were induced approximately 40-fold and 30-fold, respectively, after 4-days postinfection. The hlyF expression increased approximately threefold after 4-days postinfection. hlyG was induced approximately fivefold after 4 and 8 days postinfection. The hlyIII mRNA levels had a steady increase of approximately four-, eight-, and 12-fold following 1, 4, and 8 days postinfection, respectively. These findings suggest that B. fragilis hemolysins are induced and differentially regulated in vivo. Both parent and hlyBA mutant strains reached levels of approximately 3-8 × 10(9) cfu/mL after 1 day postinfection. However, the hlyBA mutant strain lost 2 logs in viable cell counts compared with the parent strain after 8 days postinfection. This is the first study showing HlyBA is a virulence factor which plays a role in B. fragilis survival in an intra-abdominal abscess model.
AuthorsLeandro A Lobo, Audrey L Jenkins, C Jeffrey Smith, Edson R Rocha
JournalMicrobiologyOpen (Microbiologyopen) Vol. 2 Issue 2 Pg. 326-37 (Apr 2013) ISSN: 2045-8827 [Electronic] England
PMID23441096 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Copyright© 2013 The Authors. Published by Blackwell Publishing Ltd.
Chemical References
  • Bacterial Proteins
  • Carrier Proteins
  • Hemolysin Proteins
  • Hlyb protein, Bacteria
  • RNA, Bacterial
  • RNA, Messenger
  • Abdominal Abscess (microbiology, pathology)
  • Animals
  • Bacterial Load
  • Bacterial Proteins (genetics, metabolism)
  • Bacteroides fragilis (genetics, pathogenicity)
  • Carrier Proteins (genetics, metabolism)
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Hemolysin Proteins (genetics, metabolism)
  • Intraabdominal Infections (microbiology, pathology)
  • Male
  • Microbial Viability
  • RNA, Bacterial (genetics)
  • RNA, Messenger (genetics, metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Real-Time Polymerase Chain Reaction
  • Transcription, Genetic

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: