Extracellular acidosis modulates the endocytosis and maturation of macrophages.

Abstract

Extracellular acidosis is involved in various pathological situations of central nervous system and the effects are largely mediated by acid sensing ion channels (ASICs). However, it remains unclear whether extracellular acidosis affects immune cells. Macrophages are immune cells that play important role in immune reactions. In this study we investigated the impact of extracellular acidosis on the function of bone marrow derived macrophages (BMMs). The results showed that extracellular acidosis upregulated the endocytosis, surface molecular expression and interleukin-10 secretion of BMMs, in which the expression of ASIC1 and ASIC3 was detected. Notably, extracellular acidosis stimulated endocytosis and upregulation of surface molecules expression in BMMs could be abolished by amiloride, a blocker of ASICs, and nonsteroid anti-inflammatory drugs. Our findings provide new insight into the role of extracellular acidosis in the regulation of immune function and suggest ASICs as new targets for the modulation of immune response.

• Authors: Xiaoling Kong, Xianying Tang, Wenjiao Du, Jing Tong, Yutao Yan, Fang Zheng, Min Fang, Feili Gong, Zheng Tan
• Journal: Cellular immunology (Cell Immunol) Vol. 281 Issue 1 Pg. 44-50 (Jan 2013) ISSN: 1090-2163 [Electronic] Netherlands
• PMID: 23435349 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• ASIC1 protein, human
• ASIC3 protein, human
• Acid Sensing Ion Channel Blockers
• Acid Sensing Ion Channels
• Interleukin-10
• Amiloride

Topics

• Acid Sensing Ion Channel Blockers (pharmacology)
• Acid Sensing Ion Channels (drug effects, metabolism)
• Acidosis (metabolism)
• Amiloride (pharmacology)
• Animals
• Bone Marrow Cells (metabolism)
• Cells, Cultured
• Endocytosis (immunology)
• Female
• Interleukin-10 (metabolism)
• Macrophages (metabolism)
• Mice
• Mice, Inbred C57BL