Increased expression of the antiapoptotic protein MCL1 in canine mast cell tumors.

Abstract

Myeloid cell leukemia sequence 1 (MCL1) is a potent antiapoptotic protein that plays a critical role in cell survival and drug resistance in various cancers. However, to the best of our knowledge, the role of MCL1 in mast cell tumors (MCTs) has not been investigated in dogs. Here, we detected increased MCL1 expression in MCT cell lines, regardless of the presence of a c-kit mutation. MCL1 expression increased when the cells were exposed to specific inhibitors of mitogen-activated protein kinase or Janus kinase-signaling pathways, thus protecting the cells from apoptosis, but not when KIT or phosphatidylinositol-3 kinase signaling cascades were inhibited. These results indicate that MCL1 expression may contribute to MCT survival and confer drug resistance.

• Authors: Yosuke Amagai, Akane Tanaka, Akira Matsuda, Kumiko Oida, Kyungsook Jung, Sho Nishikawa, Hyosun Jang, Saori Ishizaka, Hiroshi Matsuda
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 75 Issue 7 Pg. 971-4 (Jul 31 2013) ISSN: 1347-7439 [Electronic] Japan
• PMID: 23428776 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzamides
• Chromones
• Flavonoids
• Morpholines
• Myeloid Cell Leukemia Sequence 1 Protein
• Piperazines
• Pyrimidines
• Tyrphostins
• alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide
• 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
• Imatinib Mesylate
• Janus Kinases
• Extracellular Signal-Regulated MAP Kinases
• 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
• Sirolimus

Topics

• Animals
• Benzamides (pharmacology)
• Chromones (pharmacology)
• Dog Diseases (metabolism)
• Dogs
• Electrophoresis, Polyacrylamide Gel (veterinary)
• Extracellular Signal-Regulated MAP Kinases (antagonists & inhibitors)
• Flavonoids (pharmacology)
• Gene Expression Regulation (drug effects, physiology)
• Imatinib Mesylate
• Janus Kinases (antagonists & inhibitors)
• Mastocytoma (metabolism, veterinary)
• Morpholines (pharmacology)
• Myeloid Cell Leukemia Sequence 1 Protein (metabolism)
• Piperazines (pharmacology)
• Pyrimidines (pharmacology)
• Sirolimus (pharmacology)
• Tyrphostins (pharmacology)