Abstract |
During injury to the central nervous system (CNS), astrocytes and microglia proliferate and migrate around the lesion sites. Recently, it has been reported that one of the water channels, aquaporin-4 (AQP4) is seemed to have a role in astroglial migration and glial scar formation caused by brain injury, although its molecular mechanism is largely unknown. In the present study, we examined the expression profiles in wild-type (WT) and AQP4-deficient (AQP4/KO) mice after a stab wound to the cerebral cortex. Three days after the stab wound, AQP4 expression was observed in activated microglia around the lesion site as well as in astrocytes. A microarray analysis revealed that 444 genes around the lesion site were upregulated 3 days after the wounding in WT mice. Surprisingly, most of these up-regulations were significantly attenuated in AQP4/KO mice. Real-time RT-PCR and immunofluorescence showed that osteopontin (OPN) expression around the lesion site was much lower in AQP4/KO mice than in WT mice. Moreover, the up-regulation of pro-inflammatory cytokines was significantly attenuated in AQP4/KO mice. Taken together, these results suggest that AQP4 plays an important role in immunological function in concert with OPN under pathological conditions in the CNS.
|
Authors | Hiroko Ikeshima-Kataoka, Yoichiro Abe, Takaya Abe, Masato Yasui |
Journal | Molecular and cellular neurosciences
(Mol Cell Neurosci)
Vol. 56
Pg. 65-75
(Sep 2013)
ISSN: 1095-9327 [Electronic] United States |
PMID | 23428384
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Aqp4 protein, mouse
- Aquaporin 4
- Osteopontin
|
Topics |
- Animals
- Aquaporin 4
(genetics, metabolism)
- Astrocytes
(metabolism)
- Cerebral Cortex
(injuries, metabolism, pathology)
- Gene Expression Profiling
- Inflammation
(genetics, metabolism)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Microglia
(metabolism)
- Osteopontin
(genetics, metabolism)
- Up-Regulation
- Wounds, Stab
(immunology, metabolism, pathology)
|