Abstract |
A variation of the mouse lymphoma (L5178Y TK+/(-)-3.7.2c) assay has been developed using a microtiter cloning technique instead of the standard agar method. The cell line has been used to detect both gene mutations (at the Na+/K+ ATPase and thymidine kinase loci) and chromosome damage (micronucleus induction) in the same experiment. The system was validated using gamma-irradiation (a known clastogen), 2 direct-acting mutagens, ethyl and methyl methanesulphonate and an indirect-acting mutagen, benzo[a]pyrene. Using the assay, 1-methoxy-1,3,5-cycloheptatriene was shown to be a clastogenic mutagen in the presence of S9, since a clear dose-dependent increase in micronuclei was observed, mainly small colony thymidine kinase mutants were observed, and no ouabain-resistant mutants were induced, a profile very similar to gamma-irradiation. The results suggest that metabolic activation potential explains the results in the accompanying paper (Asquith et al., 1990). The implications for mutagenicity testing are discussed.
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Authors | J Cole, M C Diot, F N Richmond, B A Bridges |
Journal | Mutation research
(Mutat Res)
Vol. 230
Issue 1
Pg. 81-91
(May 1990)
ISSN: 0027-5107 [Print] Netherlands |
PMID | 2342500
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Cycloheptanes
- Mutagens
- 1-methoxy-1,3,5-cycloheptatriene
- Benzo(a)pyrene
- Ethyl Methanesulfonate
- Methyl Methanesulfonate
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Topics |
- Animals
- Benzo(a)pyrene
(toxicity)
- Chromosome Aberrations
- Chromosomes
(drug effects, radiation effects)
- Cloning, Molecular
- Cycloheptanes
(toxicity)
- DNA Damage
- Ethyl Methanesulfonate
(toxicity)
- Genes
(drug effects)
- Lymphoma
- Methyl Methanesulfonate
(toxicity)
- Mice
- Micronucleus Tests
- Mutagens
- Mutation
- Tumor Cells, Cultured
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