Effect of acetylation on monoclonal antibody ZCE-025 Fab': distribution in normal and tumor-bearing mice.

Abstract

Studies were performed to determine in vitro and in vivo effects of acetylation on Fab' fragments of ZCE-025, a monoclonal anti-CEA antibody. Isoelectric focusing revealed a drop in isoelectric point of 1.7 pI units following acetylation. Biodistribution studies of acetylated and nonacetylated [111In]Fab' were performed in normal BALB/c mice and in nude mice bearing the T-380 CEA-producing human colon tumor. The acetylated fragments remained in the vascular compartment longer and had significantly diminished renal uptake of 111In compared to controls. While acetylation itself effected a 50% drop in immunoreactivity, tumor uptake of the acetylated and nonacetylated 111In-labeled Fab' fragments was comparable, with the exception of one data point, through 72 h.

• Authors: J P Tarburton, S E Halpern, P L Hagan, E Sudora, A Chen, D M Fridman, A E Pfaff
• Journal: Journal of biological response modifiers (J Biol Response Mod) Vol. 9 Issue 2 Pg. 221-30 (Apr 1990) ISSN: 0732-6580 [Print] United States
• PMID: 2341861 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Carcinoembryonic Antigen
• Immunoglobulin Fab Fragments
• Indium Radioisotopes

Topics

• Acetylation
• Animals
• Antibodies, Monoclonal (pharmacokinetics)
• Carcinoembryonic Antigen (immunology)
• Immunoglobulin Fab Fragments (pharmacokinetics)
• Indium Radioisotopes (administration & dosage, metabolism)
• Isoelectric Focusing
• Kidney (metabolism)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasms, Experimental (metabolism)
• Tissue Distribution