Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice.

Abstract

This study aimed to demonstrate protective activities of the narirutin fraction from peels of Citrus unshiu against ethanol-induced hepatic damage through an animal study. Citrus narirutin fraction (CNF), contained 75% of narirutin, was obtained by an ultra-sonicated extraction and further purification. ICR mice were divided into four groups; normaldiet control, ethanol control (6.5g ethanol/kg), low-CNF (ethanol+150mg CNF/kg) and high-CNF (ethanol+300mg CNF/kg) groups. Consumption of alcohol for 8weeks induced severe liver damage with increases in prognostic indicators such as aspartate transaminase, alanine transaminase in serum whereas co-administration of CNF suppressed their increases. Excessive accumulations in liver TG and TC in ethanol control group were also suppressed by co-administration of CNF. Co-administration of CNF maintained SOD activity, GSH and malondialdehyde levels close to those of the normal diet group. Chronic consumption of alcohol also stimulated abrupt increases in pro-inflammatory cytokines such as nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver otherwise co-administration of CNF effectively suppressed production of these cytokines dose-dependently. These results indicate that co-administration of CNF with alcohol can alleviate alcohol induced liver damage through preventing lipid formation, protecting antioxidant system and suppressing productions of pro-inflammatory cytokines.

• Authors: Ho-Young Park, Sang Keun Ha, Hyojin Eom, Inwook Choi
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 55 Pg. 637-44 (May 2013) ISSN: 1873-6351 [Electronic] England
• PMID: 23416143 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Disaccharides
• Flavanones
• Interleukin-1beta
• NF-kappa B
• Tumor Necrosis Factor-alpha
• narirutin
• Malondialdehyde
• Superoxide Dismutase
• Glutathione

Topics

• Animals
• Body Weight (drug effects)
• Citrus (chemistry)
• Disaccharides (isolation & purification, pharmacology, therapeutic use)
• Flavanones (isolation & purification, pharmacology, therapeutic use)
• Glutathione (metabolism)
• Interleukin-1beta (biosynthesis)
• Liver (drug effects)
• Liver Diseases, Alcoholic (drug therapy, enzymology, metabolism)
• Male
• Malondialdehyde (metabolism)
• Mice
• Mice, Inbred ICR
• NF-kappa B (biosynthesis)
• Organ Size (drug effects)
• Superoxide Dismutase (metabolism)
• Tumor Necrosis Factor-alpha (biosynthesis)