Sophoricoside isolated from Sophora japonica ameliorates contact dermatitis by inhibiting NF-κB signaling in B cells.

Abstract

Sophoricoside (SOPH) is an isoflavone isolated from Sophora japonica (Leguminosae). In this study, the inhibitory effect of SOPH on contact dermatitis was investigated. At dosages of 3 and 10 mg/kg, SOPH ameliorated 2,4-dinitrochlorobenzene-induced acute and chronic contact dermatitis by 50-70%. As cellular targets, SOPH mainly affected the functions of B cells rather than T cells, macrophages and dendritic cells. As signaling targets, SOPH inhibited the phosphorylation and degradation of IκBα/β and the nuclear translocation of NF-κB p65 in B cells, but not in dendritic cells and macrophages. SOPH did not affect the phosphorylation of ERK, p38, and JNK MAPKs, in B cells, dendritic cells, and macrophages. Taken together, these results suggest that SOPH ameliorates contact dermatitis by inhibiting mainly NF-κB signaling in B cells.

• Authors: Hong Kyung Lee, Hyung Sook Kim, Yeon Jin Kim, Ji Sung Kim, Yoon Soo Park, Jong Soon Kang, Dong Yeon Yuk, Jin Tae Hong, Youngsoo Kim, Sang-Bae Han
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 15 Issue 3 Pg. 467-73 (Mar 2013) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 23415872 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Benzopyrans
• Dinitrochlorobenzene
• NF-kappa B
• sophoricoside

Topics

• Active Transport, Cell Nucleus (drug effects)
• Animals
• B-Lymphocytes (drug effects, immunology)
• Benzopyrans (administration & dosage, chemistry, isolation & purification)
• Cell Nucleus (metabolism)
• Cells, Cultured
• Dendritic Cells (drug effects, immunology)
• Dermatitis, Contact (drug therapy, immunology)
• Dinitrochlorobenzene (immunology)
• Macrophages (drug effects, immunology)
• Mice
• Mice, Inbred BALB C
• NF-kappa B (metabolism)
• Signal Transduction (drug effects)
• Sophora (chemistry)