Abstract |
The authors discuss here rotavirus-like particle vaccines as an alternative approach to oral live-attenuated rotavirus vaccine and their efficacy in a gnotobiotic pig model. Rotavirus virus-like particles (VLPs) were evaluated in different doses, and routes of administration, and combined with live-attenuated virus and adjuvants. A VLP vaccine composed of rotavirus VP2 and VP6 was immunogenic in gnotobiotic pigs when inoculated intranasally; however, this vaccine failed to confer protection. A combination of oral attenuated human rotavirus and intranasal 2/6VLP vaccines conferred immunogenicity, partial protection against a human rotavirus challenge and induced IFN-γ-producing T cells in the ileum of pigs with similar frequencies to human rotavirus infection. Vaccination through a combination of mucosal inductive sites and live- attenuated vaccine combined with VLP vaccines was the most effective regimen, compared with the use of a single route or a single vaccine alone. However, if formulated with neutralizing antigens, VLP vaccines may constitute a better approach in a high maternal antibody scenario.
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Authors | Marli P Azevedo, Anastasia N Vlasova, Linda J Saif |
Journal | Expert review of vaccines
(Expert Rev Vaccines)
Vol. 12
Issue 2
Pg. 169-81
(Feb 2013)
ISSN: 1744-8395 [Electronic] England |
PMID | 23414408
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Review)
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Chemical References |
- Antigens, Viral
- Capsid Proteins
- Rotavirus Vaccines
- VP2 protein, Rotavirus
- VP6 protein, Rotavirus
- Vaccines, Virus-Like Particle
- Interferon-gamma
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Topics |
- Administration, Intranasal
- Administration, Oral
- Animals
- Animals, Newborn
- Antigens, Viral
(genetics, immunology)
- Capsid Proteins
(genetics, immunology)
- Disease Models, Animal
- Germ-Free Life
- Interferon-gamma
(metabolism)
- Rotavirus Infections
(immunology, prevention & control)
- Rotavirus Vaccines
(administration & dosage, genetics, immunology)
- Swine
- T-Lymphocytes
(immunology)
- Vaccines, Virus-Like Particle
(administration & dosage, genetics, immunology)
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