Paeonol suppresses lipopolysaccharide-induced inflammatory cytokines in macrophage cells and protects mice from lethal endotoxin shock.

Abstract

Paeonol (2'-hydroxy-4'-methoxyacetophenone) is the main phenolic compound of the radix of Paeonia suffruticosa which has been used as traditional Chinese medicine. In this study, we primarily investigated the anti-inflammatory effects and the underlying mechanisms of paeonol in RAW macrophage cells; and based on these effects, we assessed the protective effects of paeonol on lipopolysaccharide-induced endotoxemia in mice. The in vitro study showed that paeonol regulated the production of TNF-α, IL-1β, IL-6, and IL-10 via inactivation of IκBα, ERK1/2, JNK, and p38 MAPK. In mouse model of lipopolysaccharide-induced endotoxemia, pro- and anti-inflammatory cytokines are significantly regulated, and thus the survival rates of lipolysaccharide-challenged mice are improved by paeonol (150, 200, or 250 mg/kg). Therefore, paeonol has a beneficial activity against lipopolysaccharide-induced inflammation in RAW 264.7 cell and mouse models.

• Authors: Na Chen, Dianfeng Liu, Lanan Wassy Soromou, Jingjing Sun, Weiting Zhong, Weixiao Guo, Meixia Huo, Hongyu Li, Shuang Guan, Zhenwen Chen, Haihua Feng
• Journal: Fundamental & clinical pharmacology (Fundam Clin Pharmacol) Vol. 28 Issue 3 Pg. 268-76 (Jun 2014) ISSN: 1472-8206 [Electronic] England
• PMID: 23413967 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 The Authors Fundamental and Clinical Pharmacology © 2013 Société Française de Pharmacologie et de Thérapeutique.

Chemical References

• Acetophenones
• Anti-Inflammatory Agents, Non-Steroidal
• Cytokines
• Lipopolysaccharides
• lipopolysaccharide, E coli O55-B5
• paeonol

Topics

• Acetophenones (administration & dosage, isolation & purification, pharmacology, therapeutic use)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, isolation & purification, pharmacology, therapeutic use)
• Cell Culture Techniques
• Cell Line
• Cell Survival (drug effects)
• Cytokines (antagonists & inhibitors, blood, immunology)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Female
• Lipopolysaccharides (toxicity)
• Macrophages (drug effects, immunology)
• Mice, Inbred C57BL
• Paeonia (chemistry)
• Plant Roots (chemistry)
• Shock, Septic (blood, immunology, prevention & control)