Abstract | OBJECTIVES: DESIGN AND METHODS:
Insulin resistance was induced by 2 months high fat diet (HFD). In vitro effects of glucose-dependent insulinotropic polypeptide (GIP) were assessed in adipose tissue explants and stromal vascular fraction (SVF). RESULTS: HFD-fed DPP4-rats gained significantly more weight and visceral fat mass, yet were more insulin sensitive. Adipose tissue of DPP4- rats demonstrated increased adipocyte maturation and increased expression of enzymes involved in triglyceride uptake and synthesis, yet increased adiponectin mRNA, reduced mRNA of proinflammatory cytokines and reduced vascular adhesion molecules, suggesting reduced inflammation. In vitro and in vivo experiments explored the role of GIP in inducing this phenotype. Indeed, we demonstrated that GIP directly enhanced adiponectin expression in rat and human adipose tissue explants and in SVF. Lastly, GIP administration to normal or HFD-fed rats elevated serum adiponectin and improved their glucose tolerance test. CONCLUSION:
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Authors | Shani Ben-Shlomo, Isabel Zvibel, Chen Varol, Lior Spektor, Amir Shlomai, Erwin M Santo, Zamir Halpern, Ran Oren, Sigal Fishman |
Journal | Obesity (Silver Spring, Md.)
(Obesity (Silver Spring))
Vol. 21
Issue 11
Pg. 2331-41
(Nov 2013)
ISSN: 1930-739X [Electronic] United States |
PMID | 23408696
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 The Obesity Society. |
Chemical References |
- Lipids
- Gastric Inhibitory Polypeptide
- Dipeptidyl Peptidase 4
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Topics |
- Adipocytes
(physiology)
- Adipogenesis
(genetics)
- Adipose Tissue
(metabolism, pathology)
- Animals
- Dipeptidyl Peptidase 4
(genetics)
- Gastric Inhibitory Polypeptide
(physiology)
- Humans
- Insulin Resistance
(genetics)
- Lipid Metabolism
(genetics)
- Lipids
(blood)
- Male
- Panniculitis
(genetics, metabolism)
- Rats
- Rats, Inbred F344
- Rats, Transgenic
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