Abstract | UNLABELLED: OBJECTIVE: DESIGN AND METHODS: The tissue levels of protein sulfenic acids (PSOH) and PSSG in liver, visceral adipose tissue, and skeletal muscle derived from glucose intolerant, obese-prone Sprague-Dawley rats were examined. RESULTS: The data in this study indicate that decreases in PSSG content occurred in liver (44%) and adipose (26%) but not skeletal muscle in obese rats that were fed a 45% fat-calorie diet versus lean rats that were fed a 10% fat-calorie diet. PSOH content did not change in the tissue between the two groups. The activity of the enzyme glutaredoxin (GLRX) responsible for reversal of PSSG formation did not change in muscle and liver between the two groups. However, levels of GLRX1 were elevated 70% in the adipose tissue of the obese, 45% fat calorie-fed rats. CONCLUSION: These are the first data to link changes in S-glutathionylation and GLRX1 to adipose tissue in the obese and demonstrate that redox changes in thiol status occur in adipose tissue as a result of obesity.
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Authors | Matthew J Picklo Sr, Joseph P Idso, Matthew I Jackson |
Journal | Obesity (Silver Spring, Md.)
(Obesity (Silver Spring))
Vol. 21
Issue 2
Pg. 297-305
(Feb 2013)
ISSN: 1930-739X [Electronic] United States |
PMID | 23404913
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2012 The Obesity Society. |
Chemical References |
- Disulfides
- Glrx protein, rat
- Glutaredoxins
- Sulfenic Acids
- Sulfhydryl Compounds
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Topics |
- Animals
- Disulfides
(analysis)
- Energy Intake
- Glucose Intolerance
- Glutaredoxins
(genetics, metabolism)
- Insulin Resistance
- Intra-Abdominal Fat
(metabolism)
- Liver
(metabolism)
- Magnetic Resonance Spectroscopy
- Male
- Muscle, Skeletal
(metabolism)
- Obesity
(metabolism)
- Oxidative Stress
- Rats
- Rats, Sprague-Dawley
- Sulfenic Acids
(analysis)
- Sulfhydryl Compounds
(metabolism)
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