TLR7/8 agonists trigger immunostimulatory properties of human 6-sulfo LacNAc dendritic cells.

Abstract

Imiquimod and resiquimod represent Toll-like receptor (TLR) 7 and 8 agonists, which emerged as attractive candidates for tumor therapy. To elucidate immune cells, which mainly contribute to TLR7/8-mediated antitumoral activity, we investigated the impact of imiquimod and resiquimod on native human 6-sulfo LacNAc (slan) dendritic cells (DCs). We found that both TLR7/8 agonists significantly improve the release of various proinflammatory cytokines by slanDCs and promote their tumor-directed cytotoxic activity. Furthermore, resiquimod efficiently augmented the ability of slanDCs to stimulate T cells and natural killer cells. These results indicate that imiquimod and resiquimod trigger various immunostimulatory properties of slanDCs, which may contribute to their antitumor effects.

• Authors: Hanka Jähnisch, Rebekka Wehner, Antje Tunger, Anja Kunze, Stephanie Oehrl, Knut Schäkel, Jacques Rohayem, Martin Bornhäuser, Torsten Tonn, Michael Bachmann, Marc Schmitz
• Journal: Cancer letters (Cancer Lett) Vol. 335 Issue 1 Pg. 119-27 (Jul 10 2013) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 23402811 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013. Published by Elsevier Ireland Ltd.

Chemical References

• Aminoquinolines
• Antigens, CD
• Antineoplastic Agents
• B7-2 Antigen
• CD86 protein, human
• Cytokines
• HLA-DR Antigens
• Imidazoles
• Immunoglobulins
• Inflammation Mediators
• Membrane Glycoproteins
• TLR7 protein, human
• TLR8 protein, human
• Toll-Like Receptor 7
• Toll-Like Receptor 8
• Imiquimod
• resiquimod

Topics

• Aminoquinolines (pharmacology)
• Antigens, CD (metabolism)
• Antineoplastic Agents (pharmacology)
• B7-2 Antigen (metabolism)
• Cell Proliferation
• Coculture Techniques
• Cytokines (metabolism)
• Cytotoxicity, Immunologic (drug effects)
• Dendritic Cells (drug effects, immunology, metabolism)
• Drug Screening Assays, Antitumor
• HLA-DR Antigens (metabolism)
• HT29 Cells
• Humans
• Imidazoles (pharmacology)
• Imiquimod
• Immunoglobulins (metabolism)
• Immunomodulation (drug effects)
• Inflammation Mediators (metabolism)
• K562 Cells
• Killer Cells, Natural (immunology)
• Lymphocyte Activation
• Membrane Glycoproteins (metabolism)
• T-Lymphocytes (immunology)
• Toll-Like Receptor 7 (agonists, metabolism)
• Toll-Like Receptor 8 (agonists, metabolism)
• CD83 Antigen