Flow cytometric evaluation of Thomsen-Friedenreich antigen on transitional cell cancer using monoclonal antibody.

Abstract

In 31 transitional cell cancer (TCC) tissues and 5 normal bladder mucosae (NBM), we compared the results of flow cytometry (FCM) and immunohistochemical examination in evaluating the expression of Thomsen-Friedenreich antigen (T-Ag) using a monoclonal antibody. On immunohistochemical examination, 14 (45%) cancer tissues showed T-Ag, while 7 (23%) cancer tissues and all NBM showed only cryptic T-Ag, which was detected only after neuraminidase treatment. Ten (32%) high grade cancer tissues showed neither T-Ag nor cryptic T-Ag. ON FCM the T-Ag positive cells (TPC) and the T-Ag positive cells after neuraminidase treatment (nTPC) were counted in fresh cell suspensions. FCM was more sensitive than immunohistochemical study in detecting T-Ag. Additionally, FCM revealed that some tumors had both T-Ag and cryptic T-Ag at the same time. The ratio of nTPC to TPC was well correlated with the stage or grade of the tumor and may be a more reliable marker of TCC than the expression of T-Ag assessed by immunohistochemical techniques.

• Authors: H Oda, T Oda, H Ohoka, M Yokoyama, M Takeuchi
• Journal: Urological research (Urol Res) Vol. 18 Issue 2 Pg. 107-11 ( 1990) ISSN: 0300-5623 [Print] Germany
• PMID: 2339479 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• Antigens, Tumor-Associated, Carbohydrate
• Biomarkers, Tumor
• Disaccharides
• Thomsen-Friedenreich antigen

Topics

• Antibodies, Monoclonal
• Antigens, Neoplasm (analysis)
• Antigens, Tumor-Associated, Carbohydrate
• Biomarkers, Tumor (analysis, immunology)
• Carcinoma, Transitional Cell (immunology, pathology)
• Disaccharides (analysis, immunology)
• Flow Cytometry
• Humans
• Immunohistochemistry
• Urinary Bladder Neoplasms (immunology, pathology)