Protein kinases D2 and D3 are novel growth regulators in HCC1806 triple-negative breast cancer cells.

Abstract

AIM:

The role of protein kinase D (PKD) in the context of breast cancer cell biology is not clear.

MATERIALS AND METHODS:

The expression of PKD isoforms was assessed in various breast cancer cell lines and PKD isoform-specific siRNAs and selective inhibitors were used to study the role of PKD in breast cancer cell growth.

RESULTS:

PKD2 and PKD3 were two major isoforms expressed at the highest levels in tumorgenic HCC1806 triple-negative breast cancer cells. Silencing PKD2 or PKD3 significantly inhibited HCC1806 cell proliferation, and PKD3 silencing had a higher inhibitory effect than PKD2 silencing on cell growth and PKD-mediated signaling. HCC1806 breast cancer cells were highly responsive to PKD inhibitors but not to a general protein kinase C (PKC) inhibitor.

CONCLUSION:

We have identified PKD2 and PKD3, especially PKD3, as novel cell growth regulators in HCC1806 triple-negative breast cancer cells. Targeting PKD instead of all PKCs effectively inhibited cell proliferation in a number of breast cancer cell lines.

• Authors: Qin Hao, Raymond McKenzie, Huachen Gan, Hua Tang
• Journal: Anticancer research (Anticancer Res) Vol. 33 Issue 2 Pg. 393-9 (Feb 2013) ISSN: 1791-7530 [Electronic] Greece
• PMID: 23393329 (Publication Type: Journal Article)

Chemical References

• Isoenzymes
• Protein Kinase D2
• Protein Kinase Inhibitors
• RNA, Small Interfering
• Protein Kinases
• protein kinase C nu
• Protein Kinase C

Topics

• Blotting, Western
• Breast Neoplasms (enzymology, genetics, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Female
• Humans
• Isoenzymes
• Protein Kinase C (metabolism)
• Protein Kinase D2
• Protein Kinase Inhibitors (pharmacology)
• Protein Kinases (metabolism)
• RNA, Small Interfering
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction (drug effects, physiology)
• Transfection