Rhamnogalacturonan II is a Toll-like receptor 4 agonist that inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells.

Abstract

We evaluated the effectiveness of rhamnogalacturonan II (RG-II)-stimulated bone marrow-derived dendritic cells (BMDCs) vaccination on the induction of antitumor immunity in a mouse lymphoma model using EG7-lymphoma cells expressing ovalbumin (OVA). BMDCs treated with RG-II had an activated phenotype. RG-II induced interleukin (IL)-12, IL-1β, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) production during dendritic cell (DC) maturation. BMDCs stimulated with RG-II facilitate the proliferation of CD8+ T cells. Using BMDCs from the mice deficient in Toll-like receptors (TLRs), we revealed that RG-II activity is dependent on TLR4. RG-II showed a preventive effect of immunization with OVA-pulsed BMDCs against EG7 lymphoma. These results suggested that RG-II expedites the DC-based immune response through the TLR4 signaling pathway.

• Authors: Sung Nam Park, Kyung Tae Noh, Young-Il Jeong, In Duk Jung, Hyun Kyu Kang, Gil Sun Cha, Su Jung Lee, Jong Keun Seo, Dae Hwan Kang, Tae-Ho Hwang, Eun Kyung Lee, Byungsuk Kwon, Yeong-Min Park
• Journal: Experimental & molecular medicine (Exp Mol Med) Vol. 45 Pg. e8 (Feb 08 2013) ISSN: 2092-6413 [Electronic] United States
• PMID: 23392255 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acute-Phase Proteins
• Adaptor Proteins, Vesicular Transport
• Carrier Proteins
• Cytokines
• Lipopolysaccharide Receptors
• Membrane Glycoproteins
• Myeloid Differentiation Factor 88
• NF-kappa B
• Receptors, Chemokine
• TICAM-1 protein, mouse
• Toll-Like Receptor 4
• lipopolysaccharide-binding protein
• rhamnogalacturonan II
• Pectins
• Mitogen-Activated Protein Kinases

Topics

• Acute-Phase Proteins (metabolism)
• Adaptor Proteins, Vesicular Transport (metabolism)
• Animals
• Bone Marrow Cells (cytology, drug effects)
• CD8-Positive T-Lymphocytes (immunology)
• Carrier Proteins (metabolism)
• Cell Differentiation (drug effects)
• Cell Nucleus (drug effects, metabolism)
• Cell Proliferation (drug effects)
• Cytokines (biosynthesis)
• Dendritic Cells (cytology, drug effects, enzymology, immunology)
• Enzyme Activation (drug effects)
• Lipopolysaccharide Receptors (metabolism)
• Lymphocyte Activation (drug effects)
• Membrane Glycoproteins (metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Mitogen-Activated Protein Kinases (metabolism)
• Myeloid Differentiation Factor 88 (metabolism)
• NF-kappa B (metabolism)
• Neoplasms (immunology, pathology)
• Pectins (pharmacology)
• Phenotype
• Protein Transport (drug effects)
• Receptors, Chemokine (metabolism)
• Signal Transduction (drug effects)
• T-Lymphocytes, Cytotoxic (cytology, drug effects)
• Toll-Like Receptor 4 (agonists, metabolism)