Alpinate oxyphyllae fructus (Alpinia Oxyphylla Miq) extracts inhibit angiotensin-II induced cardiac apoptosis in H9c2 cardiomyoblast cells.

Abstract

Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100 µg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, pro-apoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.

• Authors: Yung-Ming Chang, Chuan-Te Tsai, Chiun-Chuang Roger Wang, Yueh-Sheng Chen, Yueh-Min Lin, Chia-Hua Kuo, Bor-Show Tzang, Ray-Jade Chen, Fuu-Jen Tsai, Chih-Yang Huang
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 77 Issue 2 Pg. 229-34 ( 2013) ISSN: 1347-6947 [Electronic] England
• PMID: 23391902 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bad protein, rat
• Benzimidazoles
• Carbocyanines
• Cardiotonic Agents
• Fluorescent Dyes
• Plant Extracts
• bcl-Associated Death Protein
• Angiotensin II
• 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine
• Cytochromes c
• Caspase 3
• Caspase 9

Topics

• Alpinia (chemistry)
• Angiotensin II (pharmacology)
• Animals
• Apoptosis (drug effects)
• Benzimidazoles
• Carbocyanines
• Cardiotonic Agents (isolation & purification, pharmacology)
• Caspase 3 (genetics, metabolism)
• Caspase 9 (genetics, metabolism)
• Cell Line
• Cytochromes c (genetics, metabolism)
• Embryo, Mammalian
• Fluorescent Dyes
• Fruit (chemistry)
• Gene Expression (drug effects)
• In Situ Nick-End Labeling
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (drug effects, metabolism)
• Myoblasts, Cardiac (cytology, drug effects, metabolism)
• Plant Extracts (isolation & purification, pharmacology)
• Rats
• bcl-Associated Death Protein (genetics, metabolism)