HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Cytotoxic and apoptotic evaluations of marine bacteria isolated from brine-seawater interface of the Red Sea.

AbstractBACKGROUND:
High salinity and temperature combined with presence of heavy metals and low oxygen renders deep-sea anoxic brines of the Red Sea as one of the most extreme environments on Earth. The ability to adapt and survive in these extreme environments makes inhabiting bacteria interesting candidates for the search of novel bioactive molecules.
METHODS:
Total 20 i.e. lipophilic (chloroform) and hydrophilic (70% ethanol) extracts of marine bacteria isolated from brine-seawater interface of the Red Sea were tested for cytotoxic and apoptotic activity against three human cancer cell lines, i.e. HeLa (cervical carcinoma), MCF-7 (Breast Adenocarcinoma) and DU145 (Prostate carcinoma).
RESULTS:
Among these, twelve extracts were found to be very active after 24 hours of treatment, which were further evaluated for their cytotoxic and apoptotic effects at 48 hr. The extracts from the isolates P1-37B and P3-37A (Halomonas) and P1-17B (Sulfitobacter) have been found to be the most potent against tested cancer cell lines.
CONCLUSION:
Overall, bacterial isolates from the Red Sea displayed promising results and can be explored further to find novel drug-like molecules. The cell line specific activity of the extracts may be attributed to the presence of different polarity compounds or the cancer type i.e. biological differences in cell lines and different mechanisms of action of programmed cell death prevalent in different cancer cell lines.
AuthorsSunil Sagar, Luke Esau, Tyas Hikmawan, Andre Antunes, Karie Holtermann, Ulrich Stingl, Vladimir B Bajic, Mandeep Kaur
JournalBMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 13 Pg. 29 (Feb 06 2013) ISSN: 1472-6882 [Electronic] England
PMID23388148 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents
  • Biological Products
  • Salts
  • brine
Topics
  • Antineoplastic Agents (pharmacology, therapeutic use)
  • Apoptosis (drug effects)
  • Bacteria
  • Biological Products (pharmacology, therapeutic use)
  • Breast Neoplasms (drug therapy)
  • Cell Line, Tumor
  • Ecosystem
  • Female
  • Halomonas
  • HeLa Cells
  • Humans
  • Male
  • Neoplasms (drug therapy)
  • Oceans and Seas
  • Prostatic Neoplasms (drug therapy)
  • Salts
  • Seawater
  • Uterine Cervical Neoplasms (drug therapy)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: