Humulone suppresses replication of respiratory syncytial virus and release of IL-8 and RANTES in normal human nasal epithelial cells.

Abstract

Respiratory syncytial virus (RSV) is the major infectious agent causing serious respiratory tract inflammation in infants and young children. However, an effective vaccine and anti-viral therapy for RSV infection have not yet been developed. Hop-derived bitter acids have potent pharmacological effects on inflammation. Therefore, we investigated the effects of humulone, which is the main constituent of hop bitter acids, on the replication of RSV and release of the proinflammatory cytokine IL-8 and chemokine RANTES in RSV-infected human nasal epithelial cells (HNECs). We found that humulone prevented the expression of RSV/G-protein, formation of virus filaments and release of IL-8 and RANTES in a dose-dependent manner in RSV-infected HNECs. These findings suggest that humulone has protective effects against the replication of RSV, the virus assembly and the inflammatory responses in HNECs and that it is a useful biological product for the prevention and therapy for RSV infection.

• Authors: Jun Fuchimoto, Takashi Kojima, Tamaki Okabayashi, Tomoyuki Masaki, Noriko Ogasawara, Kazufumi Obata, Kazuaki Nomura, Satoshi Hirakawa, Naoyuki Kobayashi, Tatsuro Shigyo, Shin-ichi Yokota, Nobuhiro Fujii, Hiroyuki Tsutsumi, Tetsuo Himi, Norimasa Sawada
• Journal: Medical molecular morphology (Med Mol Morphol) Vol. 46 Issue 4 Pg. 203-9 (Dec 2013) ISSN: 1860-1499 [Electronic] Japan
• PMID: 23381605 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• CCL5 protein, human
• Chemokine CCL5
• Cyclohexenes
• Interleukin-8
• Terpenes
• Viral Proteins
• humulon

Topics

• Antiviral Agents (pharmacology)
• Cell Survival
• Cells, Cultured
• Chemokine CCL5 (metabolism)
• Cyclohexenes (pharmacology)
• Epithelial Cells (immunology, virology)
• Gene Expression (drug effects)
• Humans
• Interleukin-8 (metabolism)
• Nasal Mucosa (immunology, virology)
• Respiratory Syncytial Viruses (drug effects, physiology)
• Terpenes (pharmacology)
• Viral Proteins (genetics, metabolism)
• Virion (drug effects, physiology)
• Virus Assembly (drug effects)
• Virus Replication (drug effects)