Browning attenuates murine white adipose tissue expansion during postnatal development.

Abstract

During postnatal development of mice distinct white adipose tissue depots display a transient appearance of brown-like adipocytes. These brite (brown in white) adipocytes share characteristics with classical brown adipocytes including a multilocular appearance and the expression of the thermogenic protein uncoupling protein 1. In this study, we compared two inbred mouse strains 129S6sv/ev and C57BL6/N known for their different propensity to diet-induced obesity. We observed transient browning in retroperitoneal and inguinal adipose tissue depots of these two strains. From postnatal day 10 to 20 the increase in the abundance of multilocular adipocytes and uncoupling protein 1 expression was higher in 129S6sv/ev than in C57BL6/N pups. The parallel increase in the mass of the two fat depots was attenuated during this browning period. Conversely, epididymal white and interscapular brown adipose tissue displayed a steady increase in mass during the first 30 days of life. In this period, 129S6sv/ev mice developed a significantly higher total body fat mass than C57BL6/N. Thus, while on a local depot level a high number of brite cells is associated with the attenuation of adipose tissue expansion the strain comparison reveals no support for a systemic impact on energy balance. This article is part of a Special Issue entitled Brown and White Fat: From Signaling to Disease.

• Authors: D Lasar, A Julius, T Fromme, M Klingenspor
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1831 Issue 5 Pg. 960-8 (May 2013) ISSN: 0006-3002 [Print] Netherlands
• PMID: 23376694 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Apoptosis Regulatory Proteins
• Cidea protein, mouse
• Ion Channels
• Mitochondrial Proteins
• RNA, Messenger
• Ucp1 protein, mouse
• Uncoupling Protein 1
• Cox7a2 protein, mouse
• Electron Transport Complex IV

Topics

• Adipocytes (cytology, metabolism)
• Adipose Tissue, Brown (cytology, metabolism)
• Adipose Tissue, White (cytology, metabolism)
• Animals
• Animals, Newborn
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Body Composition
• Body Mass Index
• Electron Transport Complex IV (genetics, metabolism)
• Female
• Immunoenzyme Techniques
• Ion Channels (genetics, metabolism)
• Mice
• Mice, Inbred C57BL
• Mitochondrial Proteins (genetics, metabolism)
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction
• Uncoupling Protein 1