Abstract | BACKGROUND AND PURPOSE: The non-psychotropic cannabinoid cannabichromene is known to activate the transient receptor potential ankyrin-type1 (TRPA1) and to inhibit endocannabinoid inactivation, both of which are involved in inflammatory processes. We examined here the effects of this phytocannabinoid on peritoneal macrophages and its efficacy in an experimental model of colitis. EXPERIMENTAL APPROACH: KEY RESULTS: LPS caused a significant production of nitrites, associated to up-regulation of anandamide, iNOS, COX-2, CB1 receptors and down-regulation of CB2 receptors mRNA expression. Cannabichromene significantly reduced LPS-stimulated nitrite levels, and its effect was mimicked by cannabinoid receptor and TRPA1 agonists ( carvacrol and cinnamaldehyde) and enhanced by CB1 receptor antagonists. LPS-induced anandamide, iNOS, COX-2 and cannabinoid receptor changes were not significantly modified by cannabichromene, which, however, increased oleoylethanolamide levels. In vivo, cannabichromene ameliorated DNBS-induced colonic inflammation, as revealed by histology, immunohistochemistry and myeloperoxidase activity. CONCLUSION AND IMPLICATIONS:
Cannabichromene exerts anti-inflammatory actions in activated macrophages - with tonic CB1 cannabinoid signalling being negatively coupled to this effect - and ameliorates experimental murine colitis.
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Authors | B Romano, F Borrelli, I Fasolino, R Capasso, F Piscitelli, Mg Cascio, Rg Pertwee, D Coppola, L Vassallo, P Orlando, V Di Marzo, Aa Izzo |
Journal | British journal of pharmacology
(Br J Pharmacol)
Vol. 169
Issue 1
Pg. 213-29
(May 2013)
ISSN: 1476-5381 [Electronic] England |
PMID | 23373571
(Publication Type: Journal Article)
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Copyright | © 2013 The Authors. British Journal of Pharmacology © 2013 The British Pharmacological Society. |
Chemical References |
- Anti-Inflammatory Agents
- Benzenesulfonates
- Cannabinoids
- Lipopolysaccharides
- TRPA1 Cation Channel
- Transient Receptor Potential Channels
- Trpa1 protein, mouse
- dinitrobenzenesulfonic acid
- Nitric Oxide
- cannabichromene
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Benzenesulfonates
(toxicity)
- Blotting, Western
- Cannabinoids
(pharmacology)
- Chromatography, Liquid
- Colitis
(drug therapy, pathology)
- Down-Regulation
(drug effects)
- Immunohistochemistry
- Inflammation
(drug therapy, pathology)
- Lipopolysaccharides
(pharmacology)
- Macrophages, Peritoneal
(drug effects, metabolism)
- Male
- Mass Spectrometry
- Mice
- Mice, Inbred ICR
- Nitric Oxide
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
(drug effects)
- TRPA1 Cation Channel
- Transient Receptor Potential Channels
(agonists)
- Up-Regulation
(drug effects)
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