Isolation of rare cancer cells from blood cells using dielectrophoresis.

Abstract

In this study, we investigate the application of contactless dielectrophoresis (cDEP) for isolating cancer cells from blood cells. Devices with throughput of 0.2 mL/hr (equivalent to sorting 3×10(6) cells per minute) were used to trap breast cancer cells while allowing blood cells through. We have shown that this technique is able to isolate cancer cells in concentration as low as 1 cancer cell per 10(6) hematologic cells (equivalent to 1000 cancer cells in 1 mL of blood). We achieved 96% trapping of the cancer cells at 600 kHz and 300 V(RMS).

• Authors: Alireza Salmanzadeh, Michael B Sano, Hadi Shafiee, Mark A Stremler, Rafael V Davalos
• Journal: Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference (Annu Int Conf IEEE Eng Med Biol Soc) Vol. 2012 Pg. 590-3 ( 2012) ISSN: 2694-0604 [Electronic] United States
• PMID: 23365961 (Publication Type: Evaluation Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Topics

• Blood Cells (pathology)
• Breast Neoplasms (blood, pathology)
• Cell Line, Tumor
• Cell Separation (instrumentation, methods)
• Electrodes
• Electrophoresis (instrumentation, methods)
• Equipment Design
• Female
• Humans
• Microfluidic Analytical Techniques (instrumentation, methods)
• Neoplastic Cells, Circulating (pathology)