Abstract |
Burkholderia pseudomallei is the causative agent of melioidosis. Treatment of melioidosis is suboptimal and developing improved melioidosis therapies requires animal models. In this report, we exposed male BALB/c mice to various amounts of aerosolized B. pseudomallei 1026b to determine lethality. After establishing a median lethal dose (LD(50)) of 2,772 colony forming units (cfu)/animal, we tested the ability of doxycycline administered 6 hours after exposure to a uniformly lethal dose of ~20 LD(50) to prevent death and eliminate bacteria from the lung and spleens. Tissue bacterial burdens were examined by PCR analysis. We found that 100% of mice treated with doxycycline survived and B. pseudomallei DNA was not amplified from the lungs or spleens of most surviving mice. We conclude the BALB/c mouse is a useful model of melioidosis. Furthermore, the data generated in this mouse model indicate that doxycycline is likely to be effective in post-exposure prophylaxis of melioidosis.
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Authors | H Carl Gelhaus, Michael S Anderson, David A Fisher, Michael T Flavin, Ze-Qi Xu, Daniel C Sanford |
Journal | Scientific reports
(Sci Rep)
Vol. 3
Pg. 1146
( 2013)
ISSN: 2045-2322 [Electronic] England |
PMID | 23359492
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Aerosols
- Anti-Bacterial Agents
- Doxycycline
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Topics |
- Aerosols
- Animals
- Anti-Bacterial Agents
(administration & dosage)
- Bacterial Load
- Burkholderia pseudomallei
(pathogenicity)
- Disease Models, Animal
- Doxycycline
(administration & dosage)
- Lethal Dose 50
- Male
- Melioidosis
(drug therapy, microbiology, mortality)
- Mice
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