Anti-inflammatory effect of quinoline alkaloid skimmianine isolated from Ruta graveolens L.

Abstract

OBJECTIVE:

The present study evaluates the anti-inflammatory effect of the quinoline alkaloid skimmianine (SKM), isolated from Ruta graveolens L., against carrageenan-induced acute inflammation.

METHODS:

SKM at a dose of 5.0 mg/kg body weight was found to be the minimal concentration for maximal edema inhibition. Carrageenan suspension was administered into the sub-plantar tissue of the right hind paw 1 h after SKM and diclofenac (20 mg/kg) administration (i.p.). Paw edema was determined 3 h after carrageenan administration. The rats were then killed and mRNA expressions of TNF-α and IL-6, levels of PGE2 and TBARS, activities of COX-2, 5-LOX, SOD, catalase, glutathione peroxidase (GPx) and myeloperoxidase (MPO) and the level of nitrite were measured.

RESULTS:

SKM treatment resulted in a decrease in the mRNA levels of TNF-α and IL-6, which are upstream events of the inflammatory cascade. The levels of PGE2 and NO and the activities of COX-2 and 5-LOX were also significantly reduced after SKM treatment. Neutrophil infiltration, lipid peroxidation and associated oxidative stress in the paw tissue were reduced following SKM treatment.

CONCLUSION:

These results support the anti-inflammatory properties of skimmianine and its multi-targeted mechanism of action, suggesting its potential therapeutic efficacy in various inflammatory diseases.

• Authors: M Ratheesh, G Sindhu, Antony Helen
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 62 Issue 4 Pg. 367-76 (Apr 2013) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 23344232 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Plant Extracts
• Quinolines
• Thiobarbituric Acid Reactive Substances
• Nitric Oxide
• skimmianine
• Carrageenan
• Catalase
• Peroxidase
• Glutathione Peroxidase
• Cyclooxygenase 2
• Ptgs2 protein, rat
• Superoxide Dismutase
• Dinoprostone

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Carrageenan
• Catalase (metabolism)
• Cyclooxygenase 2 (metabolism)
• Dinoprostone (metabolism)
• Glutathione Peroxidase (metabolism)
• Inflammation (chemically induced, drug therapy, metabolism)
• Male
• Nitric Oxide (blood)
• Peroxidase (metabolism)
• Phytotherapy
• Plant Components, Aerial
• Plant Extracts (therapeutic use)
• Quinolines (therapeutic use)
• Rats
• Rats, Wistar
• Ruta
• Superoxide Dismutase (metabolism)
• Thiobarbituric Acid Reactive Substances (metabolism)