The binding of cadmium to crab cadmium metallothionein. A polarographic investigation.

Abstract

Differential pulse polarography was used to monitor the titration of crab (Cancer pagurus) cadmium metallothionein with additional cadmium. Scatchard analysis was used to calculate an apparent second-order dissociation constant. A mean value of 3.60 x 10(-7) M was calculated at 20 degrees C in 0.016 M-NaClO4, pH 8.0.

• Authors: A M Sparla, J Overnell
• Journal: The Biochemical journal (Biochem J) Vol. 267 Issue 2 Pg. 539-40 (Apr 15 1990) ISSN: 0264-6021 [Print] England
• PMID: 2334407 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cadmium
• Metallothionein

Topics

• Animals
• Brachyura (metabolism)
• Cadmium (metabolism)
• Kinetics
• Metallothionein (metabolism)
• Polarography (methods)
• Protein Binding