Shear stress inhibits apoptosis of ischemic brain microvascular endothelial cells.

Abstract

As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.

• Authors: Shan Tian, Yulong Bai, Lin Yang, Xinggang Wang, Yi Wu, Jie Jia, Yulian Zhu, Yong Cheng, Pengyue Zhang, Junfa Wu, Nianhong Wang, Guang Xia, Hua Liao, Yuling Zhang, Xiafeng Shen, Huixian Yu, Yongshan Hu
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 14 Issue 1 Pg. 1412-27 (Jan 11 2013) ISSN: 1422-0067 [Print] Switzerland
• PMID: 23344049 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proto-Oncogene Proteins c-bcl-2
• Receptor, TIE-2
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Apoptosis
• Blotting, Western
• Brain (blood supply)
• Brain Ischemia
• Cells, Cultured
• Endothelial Cells (metabolism)
• Gene Expression
• Male
• Membrane Potential, Mitochondrial (physiology)
• Mitochondria (metabolism, physiology)
• Proto-Oncogene Proteins c-akt (genetics, metabolism)
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• Rats, Sprague-Dawley
• Receptor, TIE-2 (genetics, metabolism)
• Regional Blood Flow
• Reverse Transcriptase Polymerase Chain Reaction
• Shear Strength
• Signal Transduction
• Stress, Mechanical